Detection of dentinal cracks using contrast-enhanced micro-computed tomography

Matthew D. Landrigan; John C. Flatley; Travis L. Turnbull; Jamie J. Kruzic; Jack L. Ferracane; Thomas J. Hilton; Ryan K. Roeder

doi:10.1016/j.jmbbm.2009.10.003

Detection of dentinal cracks using contrast-enhanced micro-computed tomography

Matthew D. Landrigan, John C. Flatley, Travis L. Turnbull, Jamie J. Kruzic, Jack L. Ferracane, Thomas J. Hilton, Ryan K. Roeder

School of Dentistry

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

A new technique using contrast enhanced micro-computed tomography (micro-CT) was developed to improve the ability to detect dentinal cracks in teeth and assess associated risks to oral health. Extracted, whole human molars that exhibited visual evidence of external cracks following extraction and machined, partially fractured elephant dentin specimens were labeled by BaSO₄ precipitation and imaged by micro-CT. Contrast-enhanced micro-CT was demonstrated in vitro to enable non-destructive, 3-D imaging of the presence, morphology and spatial location of dentinal cracks in whole human molars and machined specimens. BaSO₄ staining provided enhanced contrast for the detection of cracks that could not be detected prior to staining. Backscattered SEM micrographs showed that BaSO₄ was precipitated on the surfaces of dentinal cracks and within adjacent tubules. The new methods demonstrated in this study are expected to be useful for clinical and scientific studies investigating the etiology and treatment of dentinal cracks in teeth.

Original language	English (US)
Pages (from-to)	223-227
Number of pages	5
Journal	Journal of the Mechanical Behavior of Biomedical Materials
Volume	3
Issue number	2
DOIs	https://doi.org/10.1016/j.jmbbm.2009.10.003
State	Published - Feb 2010

ASJC Scopus subject areas

Biomaterials
Biomedical Engineering
Mechanics of Materials

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10.1016/j.jmbbm.2009.10.003

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title = "Detection of dentinal cracks using contrast-enhanced micro-computed tomography",

abstract = "A new technique using contrast enhanced micro-computed tomography (micro-CT) was developed to improve the ability to detect dentinal cracks in teeth and assess associated risks to oral health. Extracted, whole human molars that exhibited visual evidence of external cracks following extraction and machined, partially fractured elephant dentin specimens were labeled by BaSO4 precipitation and imaged by micro-CT. Contrast-enhanced micro-CT was demonstrated in vitro to enable non-destructive, 3-D imaging of the presence, morphology and spatial location of dentinal cracks in whole human molars and machined specimens. BaSO4 staining provided enhanced contrast for the detection of cracks that could not be detected prior to staining. Backscattered SEM micrographs showed that BaSO4 was precipitated on the surfaces of dentinal cracks and within adjacent tubules. The new methods demonstrated in this study are expected to be useful for clinical and scientific studies investigating the etiology and treatment of dentinal cracks in teeth.",

author = "Landrigan, {Matthew D.} and Flatley, {John C.} and Turnbull, {Travis L.} and Kruzic, {Jamie J.} and Ferracane, {Jack L.} and Hilton, {Thomas J.} and Roeder, {Ryan K.}",

note = "Funding Information: This research was partially supported by the U.S. Army Medical Research and Materiel Command (W81XWH-06-1-0196) through the Peer Reviewed Medical Research Program (PR054672). ",

year = "2010",

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doi = "10.1016/j.jmbbm.2009.10.003",

language = "English (US)",

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AU - Flatley, John C.

AU - Turnbull, Travis L.

AU - Kruzic, Jamie J.

AU - Ferracane, Jack L.

AU - Hilton, Thomas J.

AU - Roeder, Ryan K.

N1 - Funding Information: This research was partially supported by the U.S. Army Medical Research and Materiel Command (W81XWH-06-1-0196) through the Peer Reviewed Medical Research Program (PR054672).

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N2 - A new technique using contrast enhanced micro-computed tomography (micro-CT) was developed to improve the ability to detect dentinal cracks in teeth and assess associated risks to oral health. Extracted, whole human molars that exhibited visual evidence of external cracks following extraction and machined, partially fractured elephant dentin specimens were labeled by BaSO4 precipitation and imaged by micro-CT. Contrast-enhanced micro-CT was demonstrated in vitro to enable non-destructive, 3-D imaging of the presence, morphology and spatial location of dentinal cracks in whole human molars and machined specimens. BaSO4 staining provided enhanced contrast for the detection of cracks that could not be detected prior to staining. Backscattered SEM micrographs showed that BaSO4 was precipitated on the surfaces of dentinal cracks and within adjacent tubules. The new methods demonstrated in this study are expected to be useful for clinical and scientific studies investigating the etiology and treatment of dentinal cracks in teeth.

AB - A new technique using contrast enhanced micro-computed tomography (micro-CT) was developed to improve the ability to detect dentinal cracks in teeth and assess associated risks to oral health. Extracted, whole human molars that exhibited visual evidence of external cracks following extraction and machined, partially fractured elephant dentin specimens were labeled by BaSO4 precipitation and imaged by micro-CT. Contrast-enhanced micro-CT was demonstrated in vitro to enable non-destructive, 3-D imaging of the presence, morphology and spatial location of dentinal cracks in whole human molars and machined specimens. BaSO4 staining provided enhanced contrast for the detection of cracks that could not be detected prior to staining. Backscattered SEM micrographs showed that BaSO4 was precipitated on the surfaces of dentinal cracks and within adjacent tubules. The new methods demonstrated in this study are expected to be useful for clinical and scientific studies investigating the etiology and treatment of dentinal cracks in teeth.

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Detection of dentinal cracks using contrast-enhanced micro-computed tomography

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