Detection of intra-amniotic infection in a rabbit model by proteomics-based amniotic fluid analysis

Laura L. Klein; Breton C. Freitag; Ronald S. Gibbs; Ashok P. Reddy; Srinavasa R. Nagalla; Michael G. Gravett

doi:10.1016/j.ajog.2005.06.017

Detection of intra-amniotic infection in a rabbit model by proteomics-based amniotic fluid analysis

Laura L. Klein, Breton C. Freitag, Ronald S. Gibbs, Ashok P. Reddy, Srinavasa R. Nagalla, Michael G. Gravett

Research output: Contribution to journal › Article › peer-review

19 Scopus citations

Abstract

Objective: This study was undertaken to identify intra-amniotic infection caused by several different organisms in a rabbit model by using proteomics. Study design: Twenty infected and 18 uninfected amniotic fluid samples were subjected to proteomic analysis by surface-enhanced laser desorption ionization (SELDI-TOF, Ciphergen Biosystems, Fremont, Calif), 1- and 2-dimensional gel electrophoresis, and tandem mass spectrometry (MS/MS). Results: Detailed SELDI-TOF spectra revealed a constitutive 4.0 kd peak in all animals. Infected samples also displayed a signature double peak at 3.6 kd. A SELDI-TOF signature profile for intra-amniotic infection predicted positive amniotic fluid and/or fetal cultures with a sensitivity of 90% and specificity of 83%. Similar proteomic profiles were obtained regardless of the infecting organism. The 3.6 kd peak appeared to contain rabbit calgranulin C and rabbit calcyclin, members of the S100 family of calcium binding proteins. Conclusion: Amniotic fluid proteomic analysis was able to detect intra-amniotic infection in this experimental rabbit model. S100 proteins may be involved in the host inflammatory response to intra-amniotic infection.

Original language	English (US)
Pages (from-to)	1302-1306
Number of pages	5
Journal	American journal of obstetrics and gynecology
Volume	193
Issue number	4
DOIs	https://doi.org/10.1016/j.ajog.2005.06.017
State	Published - Oct 2005

Keywords

Amniotic fluid
Chorioamnionitis
Intra-amniotic infection
Preterm labor
Proteomics
Rabbit

ASJC Scopus subject areas

Obstetrics and Gynecology

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10.1016/j.ajog.2005.06.017

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title = "Detection of intra-amniotic infection in a rabbit model by proteomics-based amniotic fluid analysis",

abstract = "Objective: This study was undertaken to identify intra-amniotic infection caused by several different organisms in a rabbit model by using proteomics. Study design: Twenty infected and 18 uninfected amniotic fluid samples were subjected to proteomic analysis by surface-enhanced laser desorption ionization (SELDI-TOF, Ciphergen Biosystems, Fremont, Calif), 1- and 2-dimensional gel electrophoresis, and tandem mass spectrometry (MS/MS). Results: Detailed SELDI-TOF spectra revealed a constitutive 4.0 kd peak in all animals. Infected samples also displayed a signature double peak at 3.6 kd. A SELDI-TOF signature profile for intra-amniotic infection predicted positive amniotic fluid and/or fetal cultures with a sensitivity of 90% and specificity of 83%. Similar proteomic profiles were obtained regardless of the infecting organism. The 3.6 kd peak appeared to contain rabbit calgranulin C and rabbit calcyclin, members of the S100 family of calcium binding proteins. Conclusion: Amniotic fluid proteomic analysis was able to detect intra-amniotic infection in this experimental rabbit model. S100 proteins may be involved in the host inflammatory response to intra-amniotic infection.",

keywords = "Amniotic fluid, Chorioamnionitis, Intra-amniotic infection, Preterm labor, Proteomics, Rabbit",

author = "Klein, {Laura L.} and Freitag, {Breton C.} and Gibbs, {Ronald S.} and Reddy, {Ashok P.} and Nagalla, {Srinavasa R.} and Gravett, {Michael G.}",

note = "Funding Information: Supported in part by March of Dimes Research Grant No. 6-FY99-308. ",

year = "2005",

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doi = "10.1016/j.ajog.2005.06.017",

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T1 - Detection of intra-amniotic infection in a rabbit model by proteomics-based amniotic fluid analysis

AU - Klein, Laura L.

AU - Freitag, Breton C.

AU - Gibbs, Ronald S.

AU - Reddy, Ashok P.

AU - Nagalla, Srinavasa R.

AU - Gravett, Michael G.

N1 - Funding Information: Supported in part by March of Dimes Research Grant No. 6-FY99-308.

PY - 2005/10

Y1 - 2005/10

N2 - Objective: This study was undertaken to identify intra-amniotic infection caused by several different organisms in a rabbit model by using proteomics. Study design: Twenty infected and 18 uninfected amniotic fluid samples were subjected to proteomic analysis by surface-enhanced laser desorption ionization (SELDI-TOF, Ciphergen Biosystems, Fremont, Calif), 1- and 2-dimensional gel electrophoresis, and tandem mass spectrometry (MS/MS). Results: Detailed SELDI-TOF spectra revealed a constitutive 4.0 kd peak in all animals. Infected samples also displayed a signature double peak at 3.6 kd. A SELDI-TOF signature profile for intra-amniotic infection predicted positive amniotic fluid and/or fetal cultures with a sensitivity of 90% and specificity of 83%. Similar proteomic profiles were obtained regardless of the infecting organism. The 3.6 kd peak appeared to contain rabbit calgranulin C and rabbit calcyclin, members of the S100 family of calcium binding proteins. Conclusion: Amniotic fluid proteomic analysis was able to detect intra-amniotic infection in this experimental rabbit model. S100 proteins may be involved in the host inflammatory response to intra-amniotic infection.

AB - Objective: This study was undertaken to identify intra-amniotic infection caused by several different organisms in a rabbit model by using proteomics. Study design: Twenty infected and 18 uninfected amniotic fluid samples were subjected to proteomic analysis by surface-enhanced laser desorption ionization (SELDI-TOF, Ciphergen Biosystems, Fremont, Calif), 1- and 2-dimensional gel electrophoresis, and tandem mass spectrometry (MS/MS). Results: Detailed SELDI-TOF spectra revealed a constitutive 4.0 kd peak in all animals. Infected samples also displayed a signature double peak at 3.6 kd. A SELDI-TOF signature profile for intra-amniotic infection predicted positive amniotic fluid and/or fetal cultures with a sensitivity of 90% and specificity of 83%. Similar proteomic profiles were obtained regardless of the infecting organism. The 3.6 kd peak appeared to contain rabbit calgranulin C and rabbit calcyclin, members of the S100 family of calcium binding proteins. Conclusion: Amniotic fluid proteomic analysis was able to detect intra-amniotic infection in this experimental rabbit model. S100 proteins may be involved in the host inflammatory response to intra-amniotic infection.

KW - Amniotic fluid

KW - Chorioamnionitis

KW - Intra-amniotic infection

KW - Preterm labor

KW - Proteomics

KW - Rabbit

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Detection of intra-amniotic infection in a rabbit model by proteomics-based amniotic fluid analysis

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