@article{6eed99c93a4843fc8bf4215b54de5a51,
title = "Detection of neutralizing antibodies against multiple SARS-CoV-2 strains in dried blood spots using cell-free PCR",
abstract = "An easily implementable serological assay to accurately detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) neutralizing antibodies is urgently needed to better track herd immunity, vaccine efficacy and vaccination rates. Herein, we report the Split-Oligonucleotide Neighboring Inhibition Assay (SONIA) which uses real-time qPCR to measure the ability of neutralizing antibodies to block binding between DNA-barcoded viral spike protein subunit 1 and the human angiotensin-converting enzyme 2 receptor protein. The SONIA neutralizing antibody assay using finger-prick dried blood spots displays 91–97% sensitivity and 100% specificity in comparison to the live-virus neutralization assays using matched serum specimens for multiple SARS-CoV-2 variants-of-concern. The multiplex version of this neutralizing antibody assay, using easily collectable finger-prick dried blood spots, can be a valuable tool to help reveal the impact of age, pre-existing health conditions, waning immunity, different vaccination schemes and the emergence of new variants-of-concern.",
author = "Kenneth Danh and Karp, {Donna Grace} and Malvika Singhal and Akshaya Tankasala and David Gebhart and {de Jesus Cortez}, Felipe and Devangkumar Tandel and Robinson, {Peter V.} and David Seftel and Mars Stone and Graham Simmons and Anil Bagri and Schreiber, {Martin A.} and Andreas Buser and Andreas Holbro and Manuel Battegay and Morris, {Mary Kate} and Carl Hanson and Mills, {John R.} and Dane Granger and Theel, {Elitza S.} and Stubbs, {James R.} and Corash, {Laurence M.} and Tsai, {Cheng ting}",
note = "Funding Information: We are sincerely grateful to all patients, donors and staff who participated in this study. The study is funded in part by NIH SBIR 2R44DK110005-02 and 2RAI141118-02 to K.D., D.K., M.S., A.T., D.T., F.J.C., D.G., P.V.R., D.S. and C.T.T at Enable Biosciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The findings and conclusions in this article are those of the authors and do not necessarily represent the views or opinions of the California Department of Public Health of the California Health and Human Services Agency. Funding Information: We are sincerely grateful to all patients, donors and staff who participated in this study. The study is funded in part by NIH SBIR 2R44DK110005-02 and 2RAI141118-02 to K.D., D.K., M.S., A.T., D.T., F.J.C., D.G., P.V.R., D.S. and C.T.T at Enable Biosciences. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. The findings and conclusions in this article are those of the authors and do not necessarily represent the views or opinions of the California Department of Public Health of the California Health and Human Services Agency. Publisher Copyright: {\textcopyright} 2022, The Author(s).",
year = "2022",
month = dec,
doi = "10.1038/s41467-022-31796-1",
language = "English (US)",
volume = "13",
journal = "Nature communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",
number = "1",
}