TY - GEN

T1 - Determine scattering coefficient and anisotropy of scattering of tissue phantoms using reflectance-mode confocal microscopy

AU - Samatham, Ravikant

AU - Jacques, Steven L.

PY - 2009

Y1 - 2009

N2 - Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μs(1-g)). But separating scattering coefficient (μs) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.36 μm dia.) dispersed in both aqueous solutions.Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a functi n of depth of the focal volume, R(z) = ?exp(-μz) where ? (dimensionless, ?=1 for a mirror) is the local reflectivity and μ [cm-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ? into the optical scattering properties μs and g. The values of μs and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

AB - Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μs(1-g)). But separating scattering coefficient (μs) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.36 μm dia.) dispersed in both aqueous solutions.Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a functi n of depth of the focal volume, R(z) = ?exp(-μz) where ? (dimensionless, ?=1 for a mirror) is the local reflectivity and μ [cm-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ? into the optical scattering properties μs and g. The values of μs and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

KW - Collimated transmission

KW - Optical scattering properties

KW - Reflectance-mode confocal scanning laser microscope

KW - Tissuephantoms

UR - http://www.scopus.com/inward/record.url?scp=66649136122&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=66649136122&partnerID=8YFLogxK

U2 - 10.1117/12.809684

DO - 10.1117/12.809684

M3 - Conference contribution

AN - SCOPUS:66649136122

SN - 9780819474339

T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE

BT - Biomedical Applications of Light Scattering III

T2 - Biomedical Applications of Light Scattering III

Y2 - 24 January 2009 through 26 January 2009

ER -