Determine scattering coefficient and anisotropy of scattering of tissue phantoms using reflectance-mode confocal microscopy

Ravikant Samatham, Steven L. Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Scopus citations


Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μs(1-g)). But separating scattering coefficient (μs) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.36 μm dia.) dispersed in both aqueous solutions.Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a functi n of depth of the focal volume, R(z) = ?exp(-μz) where ? (dimensionless, ?=1 for a mirror) is the local reflectivity and μ [cm-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ? into the optical scattering properties μs and g. The values of μs and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

Original languageEnglish (US)
Title of host publicationBiomedical Applications of Light Scattering III
StatePublished - 2009
Externally publishedYes
EventBiomedical Applications of Light Scattering III - San Jose, CA, United States
Duration: Jan 24 2009Jan 26 2009

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherBiomedical Applications of Light Scattering III
Country/TerritoryUnited States
CitySan Jose, CA


  • Collimated transmission
  • Optical scattering properties
  • Reflectance-mode confocal scanning laser microscope
  • Tissuephantoms

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Atomic and Molecular Physics, and Optics
  • Radiology Nuclear Medicine and imaging


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