Development and integration of an extended embryo culture program

Phillip E. Patton, Karen Sadler-Fredd, Kenneth A. Burry, Marsha J. Gorrill, Aron Johnson, Janine M. Larson, Don P. Wolf

Research output: Contribution to journalArticlepeer-review

21 Scopus citations


Objective: To study and evaluate a sequential, extended embryo culture system.Design: Prospective study.Setting: University-affiliated IVF clinic.Patient(s): All couples who were treated between October 1997 and July 1998.Intervention(s): A standard human tubal fluid plus 10% serum substitute supplement (SSS) culture medium was used. The embryos were transferred to extended culture medium (S2 or G2) on day 3.Main Outcome Measure(s): Blastocyst formation and implantation and pregnancy rates.Result(s): Forty percent of the 20 donated cryopreserved embryos progressed to the blastocyst stage by day 6. Clinically, 7 (5.6%) of the 125 cycles did not result in a transfer. Blastocyst formation rates ranged from 33%-63% in the five study groups. Implantation rates ranged from 15%-52% and pregnancy rates ranged from 37%-75%.Conclusion(s): Extended culture to day 5 or 6 results in acceptable blastocyst formation rates, implantation rates, and pregnancy rates. Copyright (C) 1999 American Society for Reproductive Medicine.

Original languageEnglish (US)
Pages (from-to)418-422
Number of pages5
JournalFertility and sterility
Issue number3
StatePublished - Sep 1999
Externally publishedYes


  • Blastocyst
  • IVF
  • Multiple pregnancy
  • Sequential media

ASJC Scopus subject areas

  • Reproductive Medicine
  • Obstetrics and Gynecology


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