Differential effects of lysosomotropic amines and polyamines on processing and biological activity of EGF

Randall B. Widelitz, Lynn M. Matrisian, Diane H. Russell, Bruce E. Magun

Research output: Contribution to journalArticlepeer-review

8 Scopus citations


The effects of lysosomotropic amines and polyamines on rat fibroblasts were studied after the administration of epidermal growth factor (EGF) in order to determine whether the intracellular processing of EGF was important for transmission of its biological signal. Following the addition of EGF, cell cultures exhibited a dose-dependent increase in ornithine decarboxylase (ODC) activity. This increase in ODC activity was drastically reduced by both methylamine, a representative lysosomotropic amine, and putrescine, a polyamine precursor. However, inasmuch as methylamine inhibited EGF-induced DNA synthesis by greater than 50%, putrescine had no inhibitory effect. Lysosomotropic amines, but not polyamines, prevented EGF processing as evidenced by their ability to block the release of intracellular 125EGF and by their ability to inhibit the formation of the final intracellular processed product of EGF, as determined by isoelectric focusing. These data suggest that the processing of EGF is consistent with the induction of DNA synthesis and ODC activity. The cellular mechanisms involved in inhibition of ODC induction by polyamines appear to be distinct from those involved in lysosomotropic amines.

Original languageEnglish (US)
Pages (from-to)163-170
Number of pages8
JournalExperimental Cell Research
Issue number1
StatePublished - Nov 1984
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


Dive into the research topics of 'Differential effects of lysosomotropic amines and polyamines on processing and biological activity of EGF'. Together they form a unique fingerprint.

Cite this