Direct observation of kinesin stepping by optical trapping interferometry

Karel Svoboda; Christoph F. Schmidt; Bruce J. Schnapp; Steven M. Block

doi:10.1038/365721a0

Direct observation of kinesin stepping by optical trapping interferometry

Karel Svoboda, Christoph F. Schmidt, Bruce J. Schnapp, Steven M. Block

Research output: Contribution to journal › Article › peer-review

1641 Scopus citations

Abstract

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Original language	English (US)
Pages (from-to)	721-727
Number of pages	7
Journal	Nature
Volume	365
Issue number	6448
DOIs	https://doi.org/10.1038/365721a0
State	Published - 1993
Externally published	Yes

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@article{b336df91793645ae885116dcd7ff08f6,

title = "Direct observation of kinesin stepping by optical trapping interferometry",

abstract = "Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.",

author = "Karel Svoboda and Schmidt, {Christoph F.} and Schnapp, {Bruce J.} and Block, {Steven M.}",

year = "1993",

doi = "10.1038/365721a0",

language = "English (US)",

volume = "365",

pages = "721--727",

journal = "Nature",

issn = "0028-0836",

publisher = "Nature Publishing Group",

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T1 - Direct observation of kinesin stepping by optical trapping interferometry

AU - Svoboda, Karel

AU - Schmidt, Christoph F.

AU - Schnapp, Bruce J.

AU - Block, Steven M.

PY - 1993

Y1 - 1993

N2 - Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

AB - Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

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DO - 10.1038/365721a0

M3 - Article

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VL - 365

SP - 721

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JO - Nature

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Direct observation of kinesin stepping by optical trapping interferometry

Abstract

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