Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

Renny Edwin Fernandez; Yogeswaran Umasankar; Pandiaraj Manickam; Jeffrey C. Nickel; Laura R. Iwasaki; Burt K. Kawamoto; Kristen C. Todoki; Jo Anna M. Scott; Shekhar Bhansali

doi:10.1038/s41598-017-17835-8

Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

Renny Edwin Fernandez, Yogeswaran Umasankar, Pandiaraj Manickam, Jeffrey C. Nickel, Laura R. Iwasaki, Burt K. Kawamoto, Kristen C. Todoki, Jo Anna M. Scott, Shekhar Bhansali

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63 Scopus citations

Abstract

We report a disposable point-of-care sensing platform specific to salivary cortisol detection. The sensor is inkjet printed on a paper substrate with a metalloporphyrin based macrocyclic catalyst ink that can electrochemically reduce cortisol, captured by aptamer functionalized magnetic nanoparticles. The sensor consists of a thin magnet disc, aligned at the back of the electrode, in order to populate the magnetic nanoparticle bound cortisol at the sensing electrode area. Proof of concept studies were performed to detect salivary cortisol levels in human subjects with high and low risks for obstructive sleep apnea (OSA). High selectivity was observed to salivary cortisol against a background of closely related steroids.

Original language	English (US)
Article number	17992
Journal	Scientific Reports
Volume	7
Issue number	1
DOIs	https://doi.org/10.1038/s41598-017-17835-8
State	Published - Dec 1 2017
Externally published	Yes

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Fernandez, R. E., Umasankar, Y., Manickam, P., Nickel, J. C., Iwasaki, L. R., Kawamoto, B. K., Todoki, K. C., Scott, J. A. M., & Bhansali, S. (2017). Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients. Scientific Reports, 7(1), Article 17992. https://doi.org/10.1038/s41598-017-17835-8

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AU - Iwasaki, Laura R.

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Disposable aptamer-sensor aided by magnetic nanoparticle enrichment for detection of salivary cortisol variations in obstructive sleep apnea patients

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