Does cellular hydrogen peroxide diffuse or act locally?

Natalia M. Mishina, Pyotr A. Tyurin-Kuzmin, Kseniya N. Markvicheva, Alexander V. Vorotnikov, Vsevolod A. Tkachuk, Vibor Laketa, Carsten Schultz, Sergey Lukyanov, Vsevolod V. Belousov

Research output: Contribution to journalReview articlepeer-review

128 Scopus citations


Understanding of redox signaling requires data on the spatiotemporal distribution of hydrogen peroxide (H2O2) within the cell. The fluorescent reporter HyPer is a powerful instrument for H2O 2 imaging. However, rapid diffusion of HyPer throughout the nucleocytoplasmic compartment does not allow visualization of H 2O2 gradients on the micrometer scale. Here we dramatically improved the spatial resolution of H2O2 imaging by applying subcytoplasmic targeting of HyPer. The membrane-attached reporters identified "microdomains" of elevated H2O 2 levels within the cytoplasm of the cells exposed to growth factors. We demonstrate that diffusion of H2O2 across the cytoplasm was strongly limited, providing evidence that H2O 2 acts locally inside cells.

Original languageEnglish (US)
Pages (from-to)1-7
Number of pages7
JournalAntioxidants and Redox Signaling
Issue number1
StatePublished - Jan 1 2011
Externally publishedYes

ASJC Scopus subject areas

  • Biochemistry
  • Physiology
  • Molecular Biology
  • Clinical Biochemistry
  • Cell Biology


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