TY - JOUR
T1 - DoG Picker and TiltPicker
T2 - Software tools to facilitate particle selection in single particle electron microscopy
AU - Voss, N. R.
AU - Yoshioka, C. K.
AU - Radermacher, M.
AU - Potter, C. S.
AU - Carragher, B.
N1 - Funding Information:
Funding for the work was provided by NIH Grant RR23093. C.Y. received addition funding through the ARCS foundation. This research was conducted at the National Resource for Automated Molecular Microscopy that is supported by the NIH through the National Center for Research Resources P41 program (RR17573). We thank Edward Brignole and Albert Lueng for help with testing the TiltPicker program.
PY - 2009/5
Y1 - 2009/5
N2 - Solving the structure of macromolecular complexes using transmission electron microscopy can be an arduous task. Many of the steps in this process rely strongly on the aid of pre-existing structural knowledge, and are greatly complicated when this information is unavailable. Here, we present two software tools meant to facilitate particle picking, an early stage in the single-particle processing of unknown macromolecules. The first tool, DoG Picker, is an efficient and reasonably general, particle picker based on the Difference of Gaussians (DoG) image transform. It can function alone, as a reference-free particle picker with the unique ability to sort particles based on size, or it can also be used as a way to bootstrap the creation of templates or training datasets for other particle pickers. The second tool is TiltPicker, an interactive graphical interface application designed to streamline the selection of particle pairs from tilted-pair datasets. In many respects, TiltPicker is a re-implementation of the SPIDER WEB tilted-particle picker, but built on modern computer frameworks making it easier to deploy and maintain. The TiltPicker program also includes several useful new features beyond those of its predecessor.
AB - Solving the structure of macromolecular complexes using transmission electron microscopy can be an arduous task. Many of the steps in this process rely strongly on the aid of pre-existing structural knowledge, and are greatly complicated when this information is unavailable. Here, we present two software tools meant to facilitate particle picking, an early stage in the single-particle processing of unknown macromolecules. The first tool, DoG Picker, is an efficient and reasonably general, particle picker based on the Difference of Gaussians (DoG) image transform. It can function alone, as a reference-free particle picker with the unique ability to sort particles based on size, or it can also be used as a way to bootstrap the creation of templates or training datasets for other particle pickers. The second tool is TiltPicker, an interactive graphical interface application designed to streamline the selection of particle pairs from tilted-pair datasets. In many respects, TiltPicker is a re-implementation of the SPIDER WEB tilted-particle picker, but built on modern computer frameworks making it easier to deploy and maintain. The TiltPicker program also includes several useful new features beyond those of its predecessor.
KW - Cryo-electron microscopy
KW - Orthogonal tilt reconstruction
KW - Particle picking
KW - Random conical tilt
KW - TEM
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U2 - 10.1016/j.jsb.2009.01.004
DO - 10.1016/j.jsb.2009.01.004
M3 - Article
C2 - 19374019
AN - SCOPUS:63649113687
SN - 1047-8477
VL - 166
SP - 205
EP - 213
JO - Journal of Structural Biology
JF - Journal of Structural Biology
IS - 2
ER -