Double mutations of the N-ras gene in a patient with acute myelomonocytic leukemia

Shigeo Horiike, Shinichi Misawa, Hiroto Kaneko, Hiroyuki Nakai, Yutaka Ueda, Makoto Nakao, Kouichi Hirakawa, Masafumi Taniwaki, Kei Kashima

Research output: Contribution to journalArticlepeer-review

3 Scopus citations


We report a patient with acute myelomonocytic leukemia (AMMoL) who showed two independent point mutations of the N-ras gene at codons 12 and 13. Longitudinal analysis revealed that one mutation at codon 13 was detectable throughout his disease course and the other at codon 12 emerged as a second mutation 14 months after the diagnosis was made, at the refractory stage. Cloning to vector and subsequent sequencing confirmed that these mutations occurred in different alleles. Chromosome findings showed a simple abnormal karyotype at presentation and further karyotypic aberrations during his disease course, concomitantly with the second mutation of the N-ras gene. These findings revealed a close relationship among the disease progression, karyotypic evolution and a newly-appearing N-ras mutation.

Original languageEnglish (US)
Pages (from-to)205-213
Number of pages9
JournalInternational Journal of Hematology
Issue number4
StatePublished - Jun 1995
Externally publishedYes


  • Acute myelomonocytic leukemia (AMMoL)
  • Double mutations
  • Karyotypic evolution
  • N-ras gene

ASJC Scopus subject areas

  • Hematology


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