Double-negative (CD4-CD8-) lymphocytes bearing T-cell receptor α and β chains in normal human skin

V. Groh, M. Fabbi, F. Hochstenbach, R. T. Maziarz, J. L. Strominger

Research output: Contribution to journalArticlepeer-review

81 Scopus citations


Double-negative (CD4-CD8-) T-cell receptor α- and β-chain (TCRαβ)-expressing lymphocytes have been identified in human skin. Two skin-derived lymphocyte cell lines (II-1 and II-2) expressing this phenotype were obtained. They each expressed full-length TCR α- and β-chain transcripts and lacked TCR γ- and δ-chain as well as CD4 and CD8 mRNAs. These polyclonal cell lines shared one predominant TCR β-chain gene rearrangement. The CD3/TCRαβ complexes were functionally competent as evidenced by their capacity to transduce activation signals resulting in cell proliferation, lymphokine secretion, and cytolytic activity. The demonstration of CD4-CD8- TCRαβ lymphocytes as a subpopulation of the skin-associated immune system should contribute to understanding the biologic potential of this cell population as well as of this organ-specific immune system.

Original languageEnglish (US)
Pages (from-to)5059-5063
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Issue number13
StatePublished - 1989
Externally publishedYes

ASJC Scopus subject areas

  • General


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