Dynorphin-mediated antinociceptive effects of l-arginine and SIN-1 (an NO donor) in mice

Eunhee Chung, Brianna Burke, Andrew J. Bieber, Jason C. Doss, Yusuke Ohgami, Raymond M. Quock

Research output: Contribution to journalArticlepeer-review

31 Scopus citations


The antinociceptive response of mice to the amino acid l-arginine (l-ARG) has been attributed to either an opioid mechanism or a non-opioid but nitric oxide (NO)-dependent mechanism. Earlier it was reported that the mechanism of nitrous oxide-induced antinociception involved opioid components and was also dependent on brain NO. This study was designed to determine whether the antinociceptive effects of l-ARG and the NO donor 3-morpholinosydnoimine (SIN-1) might be mediated by brain mechanisms similar to those that are responsible for nitrous oxide (N2O) antinociception. l-ARG and SIN-1 were administered to mice intracerebroventricularly (i.c.v.), and antinociception was assessed using the acetic acid abdominal constriction test. Both l-ARG and SIN-1 caused dose-related antinociceptive effects that were blocked by naloxone and norbinaltorphimine. The antinociceptive effects of both SIN-1 and l-ARG were also blocked to a greater extent by i.c.v. administration of a rabbit antiserum against rat dynorphin 1-13 than an antiserum against methionine-enkephalin, suggesting that the SIN-1 and l-ARG effects may be related to stimulated release of dynorphin. The antinociceptive effect of l-ARG was antagonized by an inhibitor of neuoronal NO synthase enzyme, indicating that l-ARG had to be converted to NO for its antinociceptive action. These findings indicate that the mechanisms of antinociceptive action of l-ARG and SIN-1 are both mediated by dynorphin and dependent on NO.

Original languageEnglish (US)
Pages (from-to)245-250
Number of pages6
JournalBrain Research Bulletin
Issue number3
StatePublished - Jul 31 2006
Externally publishedYes


  • Antinociception
  • Arginine
  • Dynorphin
  • Mice
  • NO donors
  • Nitric oxide
  • Opioid

ASJC Scopus subject areas

  • Neuroscience(all)


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