TY - JOUR
T1 - Ectopic and eutopic secretion of chorionic gonadotropin and its subunits in vitro
T2 - comparison of clonal strains from carcinomas of lung and placenta
AU - Lieblich, Jeffrey M.
AU - Weintraub, Bruce D.
AU - Krauth, Gary H.
AU - Kohler, Peter O.
AU - Rabson, Alan S.
AU - Rosen, Saul W.
N1 - Funding Information:
1 Received September 11, 1975; accept~d Decem~er 2, 1975. " 2 Clinical Endocrinology Branch, National Insti~ute of Arthritis, Metabolism, and Digestive Diseases, National Institutes of Health, Public Health Service, V.S. Department of Health, Education, and Welfare, Bethesda, Md. 20014. 3 Division of Endocrinology, Baylor College of Medicine, Houston, Tex. 77025. 4 Division of Cancer Biology and Diagnosis, National Cancer Institute, National Institutes of Health. 5 We are indebted to Dr. Choo Sung Kim for originally cloning ChaGo-KI, to Dr. Thomas Waldmann for measuring a-fetoprotein, and to Ms. Ida Stotler and Ms, Frances Legallais for assistance and encouragement. We also thank Dr. Lynn Loriaux for careful review of this manuscript.
PY - 1976/5
Y1 - 1976/5
N2 - We compared rates of secretion in vitro of human chorionic gonadotropin (HCG) and its subunits α and β by established clonal cell lines of a bronchogenic carcinoma (ChaGo) and a choriocarcinoma (JEG). Clones showing the highest secretion rates of either HCG or its subunits were studied: ChaGo-K1, a new clonal strain, and ChaGo-C5 and JEG-3, two previously reported clonal lines. Cells were grown under identical conditions in the same laboratory. Hormone and subunit concentrations were measured by radioimmunoassays. ChaGo-K1 and ChaGo-C5 secreted only α-subunit whereas JEG-3 secreted only HCG. Average peak secretion rates in picomoles/day/mg protein were: for ChaGo-K1, HCG<0.3, α=290, and β<0.5; for ChaGo-C5, HCG<0.3, α=21, and β<0.5; and for JEG-3, HCG = 18, α<0.7, and β<0.5. The ChaGo-K1 secretion rate of α was greater than that of any of our previously reported ChaGo clones. Significant quantities of estradiol and progesterone accumulated in the media of all three cell lines; however, only JEG-3 secreted detectable quantities of placental lactogen. Thus under identical culture conditions, a bronchogenic carcinoma clonal line secreted only α-subunit, whereas a choriocarcinoma line secreted only HCG; these findings implied major differences in cellular control mechanisms. Moreover, the ectopic secretions of α exceeded the eutopic trophoblastic secretion of HCG, which suggested that in certain cases ectopic protein production may be even more efficient than nonectopic production.
AB - We compared rates of secretion in vitro of human chorionic gonadotropin (HCG) and its subunits α and β by established clonal cell lines of a bronchogenic carcinoma (ChaGo) and a choriocarcinoma (JEG). Clones showing the highest secretion rates of either HCG or its subunits were studied: ChaGo-K1, a new clonal strain, and ChaGo-C5 and JEG-3, two previously reported clonal lines. Cells were grown under identical conditions in the same laboratory. Hormone and subunit concentrations were measured by radioimmunoassays. ChaGo-K1 and ChaGo-C5 secreted only α-subunit whereas JEG-3 secreted only HCG. Average peak secretion rates in picomoles/day/mg protein were: for ChaGo-K1, HCG<0.3, α=290, and β<0.5; for ChaGo-C5, HCG<0.3, α=21, and β<0.5; and for JEG-3, HCG = 18, α<0.7, and β<0.5. The ChaGo-K1 secretion rate of α was greater than that of any of our previously reported ChaGo clones. Significant quantities of estradiol and progesterone accumulated in the media of all three cell lines; however, only JEG-3 secreted detectable quantities of placental lactogen. Thus under identical culture conditions, a bronchogenic carcinoma clonal line secreted only α-subunit, whereas a choriocarcinoma line secreted only HCG; these findings implied major differences in cellular control mechanisms. Moreover, the ectopic secretions of α exceeded the eutopic trophoblastic secretion of HCG, which suggested that in certain cases ectopic protein production may be even more efficient than nonectopic production.
UR - http://www.scopus.com/inward/record.url?scp=0017174541&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0017174541&partnerID=8YFLogxK
U2 - 10.1093/jnci/56.5.911
DO - 10.1093/jnci/56.5.911
M3 - Article
C2 - 62839
AN - SCOPUS:0017174541
SN - 0027-8874
VL - 56
SP - 911
EP - 917
JO - Journal of the National Cancer Institute
JF - Journal of the National Cancer Institute
IS - 5
ER -