Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies

Steven C. Kazmierczak; William J. Castellani; Frederick Van Lente; Edna D. Hodges; Barry Udls

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies

Steven C. Kazmierczak, William J. Castellani, Frederick Van Lente, Edna D. Hodges, Barry Udls

Research output: Contribution to journal › Article › peer-review

Abstract

We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

Original language	English (US)
Pages (from-to)	1638-1641
Number of pages	4
Journal	Clinical chemistry
Volume	36
Issue number	9
State	Published - 1990
Externally published	Yes

ASJC Scopus subject areas

General Medicine

Cite this

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title = "Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies",

abstract = "We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a {"}flipped{"} LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.",

author = "Kazmierczak, {Steven C.} and Castellani, {William J.} and {Van Lente}, Frederick and Hodges, {Edna D.} and Barry Udls",

year = "1990",

language = "English (US)",

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journal = "Clinical chemistry",

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T1 - Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum

T2 - In vivo and in vitro studies

AU - Kazmierczak, Steven C.

AU - Castellani, William J.

AU - Van Lente, Frederick

AU - Hodges, Edna D.

AU - Udls, Barry

PY - 1990

Y1 - 1990

N2 - We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

AB - We investigated the effect of reticulocytosis on the lactate dehydrogenase (LD; EC 1.1.1.27) isoenzyme LD1/LD2 ratio in patients with and without evidence of hemolytic disease. Analysis of sera from patients with reticulocytosis and in vivo hemolysis showed a mean LD1/LD2 ratio of 0.92 compared with a ratio of 0.69 in patients with in vivo hemolysis and normal reticulocyte counts. Determination of LD isoenzymes in erythrocyte lysate revealed significantly increased LD1/LD2 ratios for patients with marked reticulocytosis compared with those for patients with normal-to-minimal increases in reticulocytes. Finally, separation of mature erythrocytes and reticulocytes by flow cytometry revealed marked differences in the LD1/LD2 isoenyzme distribution between these two cell types. The ability of hemolysis to cause a "flipped" LD1/LD2 ratio is dependent on the proportion of the hemolyzed cells that are reticulocytes.

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SN - 0009-9147

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JO - Clinical chemistry

JF - Clinical chemistry

IS - 9

ER -

Effect of reticulocytosis on lactate dehydrogenase isoenzyme distribution in serum: In vivo and in vitro studies

Abstract

ASJC Scopus subject areas

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