Effects of endorphins on single myenteric neurons

John T. Williams; R. Alan North

doi:10.1016/0006-8993(79)90044-1

Effects of endorphins on single myenteric neurons

John T. Williams, R. Alan North

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

Methionine-enkephalin, leucine-enkephalin and ß-endorphin were applied in known concentrations to an in vitro preparation of myenteric neurons from the guinea-pig ileum. All 3 substances caused a rapid, reversible and dose-related inhibition of the firing of myenteric neurons induced and recorded with extracellular suction electrodes. This inhibition of firing occurred at low agonist concentrations (1-300 nM) and was reversed by naloxone and a benzomorphan narcotic antagonist. The inhibition persisted in solutions which were completely free of calcium ions - thus indicating that the observed effect is taking place directly on the neuron from which the recording is made, and that calcium ions are not required for this inhibitory action of endorphins on neuronal firing.

Original language	English (US)
Pages (from-to)	57-65
Number of pages	9
Journal	Brain research
Volume	165
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(79)90044-1
State	Published - Apr 6 1979
Externally published	Yes

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(79)90044-1

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title = "Effects of endorphins on single myenteric neurons",

abstract = "Methionine-enkephalin, leucine-enkephalin and {\ss}-endorphin were applied in known concentrations to an in vitro preparation of myenteric neurons from the guinea-pig ileum. All 3 substances caused a rapid, reversible and dose-related inhibition of the firing of myenteric neurons induced and recorded with extracellular suction electrodes. This inhibition of firing occurred at low agonist concentrations (1-300 nM) and was reversed by naloxone and a benzomorphan narcotic antagonist. The inhibition persisted in solutions which were completely free of calcium ions - thus indicating that the observed effect is taking place directly on the neuron from which the recording is made, and that calcium ions are not required for this inhibitory action of endorphins on neuronal firing.",

author = "Williams, {John T.} and {Alan North}, R.",

note = "Funding Information: This work was supported by US PHS Grants NS06672 and DA01730 and the Schweppe Foundation. We are grateful to Drs Morgan, May and Merz for gifts of drugs.",

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AU - Williams, John T.

AU - Alan North, R.

N1 - Funding Information: This work was supported by US PHS Grants NS06672 and DA01730 and the Schweppe Foundation. We are grateful to Drs Morgan, May and Merz for gifts of drugs.

PY - 1979/4/6

Y1 - 1979/4/6

N2 - Methionine-enkephalin, leucine-enkephalin and ß-endorphin were applied in known concentrations to an in vitro preparation of myenteric neurons from the guinea-pig ileum. All 3 substances caused a rapid, reversible and dose-related inhibition of the firing of myenteric neurons induced and recorded with extracellular suction electrodes. This inhibition of firing occurred at low agonist concentrations (1-300 nM) and was reversed by naloxone and a benzomorphan narcotic antagonist. The inhibition persisted in solutions which were completely free of calcium ions - thus indicating that the observed effect is taking place directly on the neuron from which the recording is made, and that calcium ions are not required for this inhibitory action of endorphins on neuronal firing.

AB - Methionine-enkephalin, leucine-enkephalin and ß-endorphin were applied in known concentrations to an in vitro preparation of myenteric neurons from the guinea-pig ileum. All 3 substances caused a rapid, reversible and dose-related inhibition of the firing of myenteric neurons induced and recorded with extracellular suction electrodes. This inhibition of firing occurred at low agonist concentrations (1-300 nM) and was reversed by naloxone and a benzomorphan narcotic antagonist. The inhibition persisted in solutions which were completely free of calcium ions - thus indicating that the observed effect is taking place directly on the neuron from which the recording is made, and that calcium ions are not required for this inhibitory action of endorphins on neuronal firing.

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Effects of endorphins on single myenteric neurons

Abstract

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