Effects of increased venous pressure on albumin- and IgG-excluded volumes in muscle

D. R. Bell, R. J. Mullins

Research output: Contribution to journalArticlepeer-review

25 Scopus citations


The effect of elevated venous pressure on the extravascular distribution space of albumin and γ-immunoglobulin G (IgG) in muscle was studied in anesthetized rabbits. In nine rabbits, venous pressure in one leg was increased from 8 to 28 mmHg while the other leg served as a control. Samples of plasma and prenodal muscle lymph were collected for 4 h before taking samples of the gastrocnemius muscle. Lymph was collected from a femoral lymphatic after ligating the popliteal efferent lymphatic. The extracellular and plasma spaces in the tissue samples were measured using 51Cr-labeled ethylenediaminetetraacetic acid (EDTA) and 125I-human serum albumin, respectively. The protein spaces were calculated from measurements of endogenous albumin and IgG concentrations using rocket electroimmunoassay. Albumin and IgG were both excluded from 50% of the interstitial space at lymph concentration. After venous congestion, the interstitial volume was twice that of control. Lymph flow increased from 0.82 ± 0.18 to 4.44 ± 0.64 μl/min. The extravascular mass of albumin and IgG increased. There was no change in the excluded volume fraction for albumin but a decrease in that for IgG. Changes in interstitial protein exclusion in skeletal muscle after pressure elevation may not be important in oncotic buffering of the edema.

Original languageEnglish (US)
Pages (from-to)H1044-H1049
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Issue number6
StatePublished - 1982

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)


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