TY - JOUR
T1 - Effects of myogenic precursor cells (C2C12) transplantation and low-level laser therapy on muscle repair
AU - Andreo, Lucas
AU - Mesquita-Ferrari, Raquel A.
AU - Ribeiro, Beatriz G.
AU - Benitte, Adriana
AU - de Fátima Nogueira, Tatiane
AU - França, Cristiane M.
AU - Silva, Daniela de Fátima Teixeira da
AU - Bussadori, Sandra K.
AU - Fernandes, Kristianne P.S.
AU - Corrêa, Fernanda I.
AU - Corrêa, João C.F.
N1 - Publisher Copyright:
© 2018 Wiley Periodicals, Inc.
PY - 2018/9
Y1 - 2018/9
N2 - Objective: The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and Methods: Sixty-five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages. Results: No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days. Conclusion: Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018.
AB - Objective: The aim of the present study was to evaluate the effects of myoblast inoculation in combination with photobiomodulation therapy (PBMT) on skeletal muscle tissue following injury. Materials and Methods: Sixty-five Wistar rats were divided into five groups: Control—animals not submitted to any procedure; Injury—cryoinjury of the tibialis anterior muscle; HBSS—animals submitted to cryoinjury and intramuscular Hank's Balanced Salt Solution; Injury + Cells—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation; Injury + Cells + LLLT—animals submitted to cryoinjury, followed by myogenic precursor cells (C2C12) transplantation and PBMT (780 nm, 40 mW, 3.2 J in 8 points). The periods analyzed were 1, 3, and 7 days. The tibialis anterior muscle was harvest for histological analysis, collagen analysis, and immunolabeling of macrophages. Results: No differences were found between the HBSS group and injury group. The Injury + Cells group exhibited an increase of inflammatory cells and immature fibers as well as a decrease in the number of macrophages on Day 1. The Injury + Cells + LLLT group exhibited a decrease in myonecrosis and inflammatory infiltrate at 7 days, but an increase in inflammatory infiltrate at 1 and 3 days as well as an increase in blood vessels at 3 and 7 days, an increase in macrophages at 3 days and better collagen organization at 7 days. Conclusion: Cell transplantation combined with PBMT led to an increase in the number of blood vessels, a reduction in myonecrosis and total inflammatory cells as well as better organization of collagen fibers during the skeletal muscle repair process. Lasers Surg. Med. 50:781–791, 2018.
KW - cell transplantation
KW - laser therapy
KW - myoblasts
KW - skeletal muscle
UR - http://www.scopus.com/inward/record.url?scp=85041332920&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85041332920&partnerID=8YFLogxK
U2 - 10.1002/lsm.22798
DO - 10.1002/lsm.22798
M3 - Article
AN - SCOPUS:85041332920
SN - 0196-8092
VL - 50
SP - 781
EP - 791
JO - Lasers in Surgery and Medicine
JF - Lasers in Surgery and Medicine
IS - 7
ER -