Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

Michael J. Rutten; Hwa Tzong Chern; C. Diane Moore; James Hamilton; Laurence Y. Cheung

doi:10.1016/0016-6480(89)90076-2

Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

Michael J. Rutten, Hwa Tzong Chern, C. Diane Moore, James Hamilton, Laurence Y. Cheung

Research output: Contribution to journal › Article › peer-review

Abstract

The effects of bovine parathyroid hormone (bPTH_1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH_1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH_1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH_1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH_1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH_1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH_1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH_1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

Original language	English (US)
Pages (from-to)	241-246
Number of pages	6
Journal	General and Comparative Endocrinology
Volume	75
Issue number	2
DOIs	https://doi.org/10.1016/0016-6480(89)90076-2
State	Published - Aug 1989
Externally published	Yes

ASJC Scopus subject areas

Animal Science and Zoology
Endocrinology

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10.1016/0016-6480(89)90076-2

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@article{a6971d0264cb42948d4e54716e7eecae,

title = "Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum",

abstract = "The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.",

author = "Rutten, {Michael J.} and Chern, {Hwa Tzong} and Moore, {C. Diane} and James Hamilton and Cheung, {Laurence Y.}",

note = "Funding Information: The authors thank Joyce Capps for the typing. Supported by the Veterans Administration Medical Research Service, and NIH Grant DK25998-11.",

year = "1989",

month = aug,

doi = "10.1016/0016-6480(89)90076-2",

language = "English (US)",

volume = "75",

pages = "241--246",

journal = "General and Comparative Endocrinology",

issn = "0016-6480",

publisher = "Academic Press Inc.",

number = "2",

}

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T1 - Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

AU - Rutten, Michael J.

AU - Chern, Hwa Tzong

AU - Moore, C. Diane

AU - Hamilton, James

AU - Cheung, Laurence Y.

N1 - Funding Information: The authors thank Joyce Capps for the typing. Supported by the Veterans Administration Medical Research Service, and NIH Grant DK25998-11.

PY - 1989/8

Y1 - 1989/8

N2 - The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

AB - The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

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U2 - 10.1016/0016-6480(89)90076-2

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SN - 0016-6480

VL - 75

SP - 241

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JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

IS - 2

ER -

Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

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