Electron cryo-tomography of vestibular hair-cell stereocilia

Zoltan Metlagel; Jocelyn F. Krey; Junha Song; Mark F. Swift; William J. Tivol; Rachel A. Dumont; Jasmine Thai; Alex Chang; Helia Seifikar; Niels Volkmann; Dorit Hanein; Peter G. Barr-Gillespie; Manfred Auer

doi:10.1016/j.jsb.2019.02.006

Electron cryo-tomography of vestibular hair-cell stereocilia

Zoltan Metlagel, Jocelyn F. Krey, Junha Song, Mark F. Swift, William J. Tivol, Rachel A. Dumont, Jasmine Thai, Alex Chang, Helia Seifikar, Niels Volkmann, Dorit Hanein, Peter G. Barr-Gillespie, Manfred Auer

Otolaryngology

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.

Original language	English (US)
Pages (from-to)	149-155
Number of pages	7
Journal	Journal of Structural Biology
Volume	206
Issue number	2
DOIs	https://doi.org/10.1016/j.jsb.2019.02.006
State	Published - May 1 2019

Keywords

Actin
Cryo-electron microscopy
Hair cells
Stereocilia

ASJC Scopus subject areas

Structural Biology

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10.1016/j.jsb.2019.02.006

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title = "Electron cryo-tomography of vestibular hair-cell stereocilia",

abstract = "High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.",

keywords = "Actin, Cryo-electron microscopy, Hair cells, Stereocilia",

author = "Zoltan Metlagel and Krey, {Jocelyn F.} and Junha Song and Swift, {Mark F.} and Tivol, {William J.} and Dumont, {Rachel A.} and Jasmine Thai and Alex Chang and Helia Seifikar and Niels Volkmann and Dorit Hanein and Barr-Gillespie, {Peter G.} and Manfred Auer",

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T1 - Electron cryo-tomography of vestibular hair-cell stereocilia

AU - Metlagel, Zoltan

AU - Krey, Jocelyn F.

AU - Song, Junha

AU - Swift, Mark F.

AU - Tivol, William J.

AU - Dumont, Rachel A.

AU - Thai, Jasmine

AU - Chang, Alex

AU - Seifikar, Helia

AU - Volkmann, Niels

AU - Hanein, Dorit

AU - Barr-Gillespie, Peter G.

AU - Auer, Manfred

PY - 2019/5/1

Y1 - 2019/5/1

N2 - High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.

AB - High-resolution imaging of hair-cell stereocilia of the inner ear has contributed substantially to our understanding of auditory and vestibular function. To provide three-dimensional views of the structure of stereocilia cytoskeleton and membranes, we developed a method for rapidly freezing unfixed stereocilia on electron microscopy grids, which allowed subsequent 3D imaging by electron cryo-tomography. Structures of stereocilia tips, shafts, and tapers were revealed, demonstrating that the actin paracrystal was not perfectly ordered. This sample-preparation and imaging procedure will allow for examination of structural features of stereocilia in a near-native state.

KW - Actin

KW - Cryo-electron microscopy

KW - Hair cells

KW - Stereocilia

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ER -

Electron cryo-tomography of vestibular hair-cell stereocilia

Abstract

Keywords

ASJC Scopus subject areas

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