TY - JOUR
T1 - Encephalitogenic T lymphocytes develop from SJL/J hematopoietic cells transplanted into severe combined immimodeficient ( SCID) mice
AU - Jones, Richard E.
AU - Whitham, Ruth H.
AU - Sullivan, Tim
AU - Mass, Michele
AU - Bourdette, dennis N.
N1 - Funding Information:
The authorst hank Verna Russell for preparingh is-tology slides and Carolyn Steuer for expert animal care. This work was supportedb y the US Department of VeteransA ffairs and the Medical ResearchF ounda-tion of Oregon.
PY - 1995/3
Y1 - 1995/3
N2 - Previously, we constructed chimeras by injecting hematopoietic cells from experimental autoimmune encephalomyelitis (EAE)-susceptible SJL (H-2S) strain mice into severe combined immunodeficient (SCID) C.B-17scid /scid (H-2d) mice. These SCID mouse-SJL mouse hematopoietic cell chimeras developed passive EAE following adoptive transfer of PLP S139-151-specific SJL T lymphocyte line cells, but were resistant to active EAE induced by primary immunization with PLP S139-151. In order to gain an understanding of the encephalitogenic potential of transplanted hematopoietic progenitors in SCID mouse-SJL mouse chimeras, we attempted to induce EAE in hematopoietic chimeras constructed with or without an additional SJL fetal thymus implant. Chimeras with the thymus implant were susceptible to passive and active EAE while chimeras without the thymus implant were susceptible to passive but not active EAE. Encephalitogenic, CD4+, TCR+ T lymphocytes were selected in vitro from PLP S139-151-immunized, thymus-implanted chimeras. These results showed that hematopoietic SJL progenitors developed into antigen-presenting accessory cells and immunocompetent encephalitogenic T lymphocytes following transplantation into SCID mice. The development of primary immune reactivity depended on a fetal thymus implant for expression in SCID mouse-SJL mouse chimeras.
AB - Previously, we constructed chimeras by injecting hematopoietic cells from experimental autoimmune encephalomyelitis (EAE)-susceptible SJL (H-2S) strain mice into severe combined immunodeficient (SCID) C.B-17scid /scid (H-2d) mice. These SCID mouse-SJL mouse hematopoietic cell chimeras developed passive EAE following adoptive transfer of PLP S139-151-specific SJL T lymphocyte line cells, but were resistant to active EAE induced by primary immunization with PLP S139-151. In order to gain an understanding of the encephalitogenic potential of transplanted hematopoietic progenitors in SCID mouse-SJL mouse chimeras, we attempted to induce EAE in hematopoietic chimeras constructed with or without an additional SJL fetal thymus implant. Chimeras with the thymus implant were susceptible to passive and active EAE while chimeras without the thymus implant were susceptible to passive but not active EAE. Encephalitogenic, CD4+, TCR+ T lymphocytes were selected in vitro from PLP S139-151-immunized, thymus-implanted chimeras. These results showed that hematopoietic SJL progenitors developed into antigen-presenting accessory cells and immunocompetent encephalitogenic T lymphocytes following transplantation into SCID mice. The development of primary immune reactivity depended on a fetal thymus implant for expression in SCID mouse-SJL mouse chimeras.
KW - Experimental autoimmune encephalomyelitis
KW - Hematopoietic chimeras
KW - SJL mice
KW - Severe combined immunodeficient (SCID) mice
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U2 - 10.1016/0165-5728(94)00179-R
DO - 10.1016/0165-5728(94)00179-R
M3 - Article
C2 - 7535789
AN - SCOPUS:0028897711
SN - 0165-5728
VL - 57
SP - 155
EP - 164
JO - Journal of Neuroimmunology
JF - Journal of Neuroimmunology
IS - 1-2
ER -