TY - JOUR
T1 - Endothelial cell, pericyte, and perivascular resident macrophage-type melanocyte interactions regulate cochlear intrastrial fluid-blood barrier permeability
AU - Neng, Lingling
AU - Zhang, Fei
AU - Kachelmeier, Allan
AU - Shi, Xiaorui
N1 - Funding Information:
We thank Ahmed Hassan and John Henry at life Sciences Division, Lawrence Berkeley National Laboratory, Berkeley for their input and guidance in developing workflows for 3D rendering. This work was supported by National Institutes of Health/National Institute on Deafness and Other Communication Disorders Grants DC008888-02A1 (to X.S.), DC008888-02S1 (to X.S.), R01-DC010844 (to X.S.), NIH NIDCD R21-DC12398-01 (to X.R), and NIHP30-DC005983.
PY - 2013/4
Y1 - 2013/4
N2 - The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.
AB - The integrity of the fluid-blood barrier in the stria vascularis is critical for maintaining inner ear homeostasis, especially for sustaining the endocochlear potential, an essential driving force for hearing function. However, the mechanisms that control intrastrial fluid-blood barrier permeability remain largely unknown. At the cellular level, the intrastrial fluid-blood barrier comprises cochlear microvascular endothelial cells connected to each other by tight junctions (TJs), an underlying basement membrane, and a second line of support consisting of cochlear pericytes and perivascular resident macrophage-type melanocytes. In this study, we use a newly established primary cell culture-based in vitro model to show that endothelial cells, pericytes, and perivascular resident macrophage-type melanocytes interact to control intrastrial fluid-blood barrier permeability. When the endothelial cell monolayer was treated with pericyte - or perivascular resident macrophage-type melanocyte - conditioned media, the permeability of the endothelial cell monolayer was significantly reduced relative to an untreated endothelial cell monolayer. Further study has shown the pericytes and perivascular resident macrophage-type melanocytes to regulate TJ expression in the endothelial cell monolayer. The new cell culture-based in vitro model offers a unique opportunity to obtain information on the organ-specific characteristics of the cochlear blood/tissue barrier. Our finding demonstrates the importance of signaling among pericytes, endothelial cells, and perivascular resident macrophage-type melanocytes to the integrity of the intrastrial fluid-blood barrier.
KW - endothelial cell
KW - mouse cochlea
KW - paracellular permeability
KW - pericyte
KW - perivascular resident macrophages-type melanocyte
KW - primary cell line
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U2 - 10.1007/s10162-012-0365-9
DO - 10.1007/s10162-012-0365-9
M3 - Article
C2 - 23247886
AN - SCOPUS:84879606476
SN - 1525-3961
VL - 14
SP - 175
EP - 185
JO - JARO - Journal of the Association for Research in Otolaryngology
JF - JARO - Journal of the Association for Research in Otolaryngology
IS - 2
ER -