Erratum: Genetic and Stress-Induced Loss of NG2 Glia Triggers Emergence of Depressive-like Behaviors through Reduced Secretion of FGF2 (Genetic and Stress-Induced Loss of NG2 Glia Triggers Emergence of Depressive-like Behaviors through Reduced Secretion of FGF2 (2015) 88(5) (941–956), (S0896627315009745), (10.1016/j.neuron.2015.10.046))

(Neuron 88, 941–956; December 2, 2015) In this article, certain images were shifted and ended up mismatching the original figure legends or missing their description. We also inadvertently left out two references. We apologize for these errors. The legends for Figures 3, 4, and 6 should read as follows. Figure 3. NG2 Glia Ablation Leads to Impaired Glutamate Transporter Expression and Glutamate Uptake Deficit in the PFC (A) Schematics of experimental protocol used for 3H-D-aspartate uptake assays. (B) 3H-D-aspartate uptake assayed in control and iDTR PFC gliosomes and in primary cortical astrocyte cultures at 3DT and 7DT; n = 8–10 per group. (C) Intracellular and cell membrane-bound levels of GLAST from PFC control and iDTR in primary cortical astrocyte cultures at 7DT. (D) Protein expression levels of glutamate transporter GLAST, GLT-1, and pSTAT3 in the PFCs of control and iDTR mice at 7DT. (E) Protein levels of GLAST in the hippocampus and striatum at 7DT. (F) 3H-D-aspartate uptake assayed in control and iDTR hippocampal and striatal gliosomes at 7DT. Error bars, mean ± SEM (^∗p < 0.05). n = 4–5 per group unless indicated otherwise. Figure 4. DT-Induced Ablation of NG2 Glial Cells Causes Depressive-like Behaviors in Mice (A) Open field activity. (B–D) Behavioral analyses at 7DT (n = 12–14 per group; t test; OF, open field test; EPM, elevated plus maze; SP, sucrose preference test; SI, social interaction test). (B) Center entry measures normalized to total distance traveled assessed OF. % open arm entry frequencies over all entries assessed by EPM. 1% sucrose water preference assessed by SF. (C) Schematic for subthreshold microdefeat protocol. (D) Average SI scores following microdefeat. (E) Paradigm for local NG2 glia ablation in the PFC. (F and G) Representative images (F) and cell number quantification (G) of NG2 glia in the PFC and motor cortex after NaCl or DT infusion via cannula into the PFC of iDTR mice (n = 4 per group; t test). (H) Open field activity after NaCl or DT infusion. (I) Behavioral analyses performed after acute focal NG2 ablation (n = 11–16 per group; t test). Error bars, mean ± SEM (^∗p < 0.05, ^∗∗p < 0.01). Scale bar, 40 μm. See also Figure S5. Figure 6. Reduced NG2 Density in Animal Models of Depression and MDD Subjects (A) Experimental protocol for social defeat stress paradigm (SDSP) and schematic of anatomical brain regions investigated: prefrontal cortex (PFC), prelimbic (PrL) and infralimbic (IL) regions of the CA1 region of hippocampus. (B and C) Representative images (B) and cell quantification (C) of PDGFRα+ cells in CA1 and PFC at 4d, 8d, and 8d+10d of susceptible animals (SUS) compared to resilient (RES) mice (n = 5–6 per group; t test). (D) Representative images and cell number of PDGFRα+ cells in PFCs of adult congenitally non-learned helpless (cNLH) and learned helpless (cLH) rats (n = 4 per group; t test). (E) Protein levels of PDGFRα from PFCs of the control and MDD subjects. Boxplot of PDGFRα expression levels in MDD subjects and aged- and sex-matched controls (CTRL n = 8, MDD n = 12; paired t test). (F) Characterization of human NG2 glial cells in the frontal cortex gray matter characterized using PDGFRα immunostaining. Arrows, NG2 glia; Arrowheads, pericytes. (G) Representative images and cell number (% over total DAPI+ nuclei) of NG2 glia in the PFCs of control and MDD subjects. Arrows, NG2 glia (^∗p < 0.05, ^∗∗p < 0.01). Error bars, mean ± SEM. Scale bars, 40 μm; (F) 20 μm. We also omitted a description for pPKC western blot in Figure 5I. Here, the actin bands represented in Figure 5C and 5I refer to the same samples from the same SDS-PAGE run. The actin bands were used twice because pPKC expression was related to GluR1 membrane localization and therefore was represented also in Figure 5I. Figure 5 (H and I) Serine phosphorylation (H) and levels of intracellular and membrane-bound GluR1 (I) in the PFC at 21dpDT. Protein expression levels of pPKC in the PFC of control and iDTR at 21dpDT. Note that pPKC was blotted on the same SDS-PAGE run as pSTAT3, GLAST, and GLT-1 shown in 5C; therefore, they share the same actin blot. Finally, in our final published manuscript, we omitted the references Fournier et al. (2004) and Sheldon et al. (2006) in the “Biotinylation of Cell Surface Markers” section of the Experimental Procedures. The information should have appeared as follows. Biotinylation of cell surface proteins in our system was performed as previously described (Davis et al., 1998; Sheldon et al., 2006).