Establishment of a tractable genetic transformation system in Veillonella spp.

Jinman Liu; Zhoujie Xie; Justin Merritt; Fengxia Qi

doi:10.1128/AEM.00196-12

Establishment of a tractable genetic transformation system in Veillonella spp.

Jinman Liu, Zhoujie Xie, Justin Merritt, Fengxia Qi

Research output: Contribution to journal › Article › peer-review

13 Scopus citations

Abstract

We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies.

Original language	English (US)
Pages (from-to)	3488-3491
Number of pages	4
Journal	Applied and Environmental Microbiology
Volume	78
Issue number	9
DOIs	https://doi.org/10.1128/AEM.00196-12
State	Published - May 2012
Externally published	Yes

ASJC Scopus subject areas

Biotechnology
Food Science
Applied Microbiology and Biotechnology
Ecology

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title = "Establishment of a tractable genetic transformation system in Veillonella spp.",

abstract = "We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies.",

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AU - Xie, Zhoujie

AU - Merritt, Justin

AU - Qi, Fengxia

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AB - We have constructed the first Escherichia coli-Veillonella shuttle vector based on an endogenous plasmid (pVJL1) isolated from a clinical Veillonella strain. A highly transformable Veillonella strain was also identified. Both the shuttle vector and the transformable strain should be valuable tools for future Veillonella genetic studies.

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Establishment of a tractable genetic transformation system in Veillonella spp.

Abstract

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