TY - JOUR
T1 - Estrogen control of prolactin synthesis in vitro
AU - Lieberman, M. E.
AU - Maurer, R. A.
AU - Gorski, J.
PY - 1978
Y1 - 1978
N2 - Primary cultures of rat pituitary cells respond to estradiol - 17β by increased incorporation of radiolabeled precursors into prolactin but not into the bulk of other cellular proteins. The rate of increase in prolactin synthesis is dose dependent, reaching maximal levels in the physiological range of estradiol. At a concentration of 1 nM, estradiol, diethylstilbestrol, and estriol are stimulatory whereas androgens, progesterone, and corticosterone are without significant effect. Exposure of pituitary cells to 10 nM estradiol resulted in a 500% increase in prolactin synthesis after 7 days of culture. The results indicate that estradiol can stimulate prolactin synthesis through direct action on the pituitary.
AB - Primary cultures of rat pituitary cells respond to estradiol - 17β by increased incorporation of radiolabeled precursors into prolactin but not into the bulk of other cellular proteins. The rate of increase in prolactin synthesis is dose dependent, reaching maximal levels in the physiological range of estradiol. At a concentration of 1 nM, estradiol, diethylstilbestrol, and estriol are stimulatory whereas androgens, progesterone, and corticosterone are without significant effect. Exposure of pituitary cells to 10 nM estradiol resulted in a 500% increase in prolactin synthesis after 7 days of culture. The results indicate that estradiol can stimulate prolactin synthesis through direct action on the pituitary.
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U2 - 10.1073/pnas.75.12.5946
DO - 10.1073/pnas.75.12.5946
M3 - Article
C2 - 282615
AN - SCOPUS:0010367326
SN - 0027-8424
VL - 75
SP - 5946
EP - 5949
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 12
ER -