Estrogen induces multiple regulatory B cell subtypes and promotes M2 microglia and neuroprotection during experimental autoimmune encephalomyelitis

Gil Benedek; Jun Zhang; Sheetal Bodhankar; Ha Nguyen; Gail Kent; Kelley Jordan; Dustin Manning; Arthur A. Vandenbark; Halina Offner

doi:10.1016/j.jneuroim.2016.02.009

Estrogen induces multiple regulatory B cell subtypes and promotes M2 microglia and neuroprotection during experimental autoimmune encephalomyelitis

Gil Benedek, Jun Zhang, Sheetal Bodhankar, Ha Nguyen, Gail Kent, Kelley Jordan, Dustin Manning, Arthur A. Vandenbark, Halina Offner

Neurology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

Sex hormones promote immunoregulatory effects on multiple sclerosis. The current study evaluated estrogen effects on regulatory B cells and resident CNS microglia during experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate an estrogen-dependent induction of multiple regulatory B cell markers indicative of IL-10 dependent as well as IFN-γ dependent pathways. Moreover, although estrogen pretreatment of EAE mice inhibited the infiltration of pro-inflammatory cells into the CNS, it enhanced the frequency of regulatory B cells and M2 microglia. Our study suggests that estrogen has a broad effect on the development of regulatory B cells during EAE, which in turn could promote neuroprotection.

Original language	English (US)
Pages (from-to)	45-53
Number of pages	9
Journal	Journal of Neuroimmunology
Volume	293
DOIs	https://doi.org/10.1016/j.jneuroim.2016.02.009
State	Published - Apr 15 2016

Keywords

EAE
Estrogen
IFN-γ
IL-10
Microglia
Regulatory B cell

ASJC Scopus subject areas

Immunology and Allergy
Immunology
Neurology
Clinical Neurology

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10.1016/j.jneuroim.2016.02.009

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title = "Estrogen induces multiple regulatory B cell subtypes and promotes M2 microglia and neuroprotection during experimental autoimmune encephalomyelitis",

abstract = "Sex hormones promote immunoregulatory effects on multiple sclerosis. The current study evaluated estrogen effects on regulatory B cells and resident CNS microglia during experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate an estrogen-dependent induction of multiple regulatory B cell markers indicative of IL-10 dependent as well as IFN-γ dependent pathways. Moreover, although estrogen pretreatment of EAE mice inhibited the infiltration of pro-inflammatory cells into the CNS, it enhanced the frequency of regulatory B cells and M2 microglia. Our study suggests that estrogen has a broad effect on the development of regulatory B cells during EAE, which in turn could promote neuroprotection.",

keywords = "EAE, Estrogen, IFN-γ, IL-10, Microglia, Regulatory B cell",

author = "Gil Benedek and Jun Zhang and Sheetal Bodhankar and Ha Nguyen and Gail Kent and Kelley Jordan and Dustin Manning and Vandenbark, {Arthur A.} and Halina Offner",

note = "Funding Information: This work was supported by NIH/NINDS grant RO1 NS080890 (H.O). This material is the result of work supported with resources and the use of facilities at the VA Portland Health Care System, Portland, OR. The contents do not represent the views of the Department of Veterans Affairs or the US government. Funding Information: This work was supported by NIH/NINDS grant RO1 NS080890 (H.O). This material is the result of work supported with resources and the use of facilities at the VA Portland Health Care System, Portland, OR. The contents do not represent the views of the Department of Veterans Affairs or the US government. Publisher Copyright: {\textcopyright} 2016 Elsevier B.V.",

year = "2016",

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doi = "10.1016/j.jneuroim.2016.02.009",

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AU - Benedek, Gil

AU - Zhang, Jun

AU - Bodhankar, Sheetal

AU - Nguyen, Ha

AU - Kent, Gail

AU - Jordan, Kelley

AU - Manning, Dustin

AU - Vandenbark, Arthur A.

AU - Offner, Halina

N1 - Funding Information: This work was supported by NIH/NINDS grant RO1 NS080890 (H.O). This material is the result of work supported with resources and the use of facilities at the VA Portland Health Care System, Portland, OR. The contents do not represent the views of the Department of Veterans Affairs or the US government. Funding Information: This work was supported by NIH/NINDS grant RO1 NS080890 (H.O). This material is the result of work supported with resources and the use of facilities at the VA Portland Health Care System, Portland, OR. The contents do not represent the views of the Department of Veterans Affairs or the US government. Publisher Copyright: © 2016 Elsevier B.V.

PY - 2016/4/15

Y1 - 2016/4/15

N2 - Sex hormones promote immunoregulatory effects on multiple sclerosis. The current study evaluated estrogen effects on regulatory B cells and resident CNS microglia during experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate an estrogen-dependent induction of multiple regulatory B cell markers indicative of IL-10 dependent as well as IFN-γ dependent pathways. Moreover, although estrogen pretreatment of EAE mice inhibited the infiltration of pro-inflammatory cells into the CNS, it enhanced the frequency of regulatory B cells and M2 microglia. Our study suggests that estrogen has a broad effect on the development of regulatory B cells during EAE, which in turn could promote neuroprotection.

AB - Sex hormones promote immunoregulatory effects on multiple sclerosis. The current study evaluated estrogen effects on regulatory B cells and resident CNS microglia during experimental autoimmune encephalomyelitis (EAE). Herein, we demonstrate an estrogen-dependent induction of multiple regulatory B cell markers indicative of IL-10 dependent as well as IFN-γ dependent pathways. Moreover, although estrogen pretreatment of EAE mice inhibited the infiltration of pro-inflammatory cells into the CNS, it enhanced the frequency of regulatory B cells and M2 microglia. Our study suggests that estrogen has a broad effect on the development of regulatory B cells during EAE, which in turn could promote neuroprotection.

KW - EAE

KW - Estrogen

KW - IFN-γ

KW - IL-10

KW - Microglia

KW - Regulatory B cell

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Estrogen induces multiple regulatory B cell subtypes and promotes M2 microglia and neuroprotection during experimental autoimmune encephalomyelitis

Abstract

Keywords

ASJC Scopus subject areas

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