TY - JOUR
T1 - Expression and regulation of tumor necrosis factor TNF and TNF-receptor family members in the macaque corpus luteum during the menstrual cycle
AU - Peluffo, Marina C.
AU - Young, Kelly A.
AU - Hennebold, Jon D.
AU - Stouffer, Richard L.
N1 - Copyright:
Copyright 2012 Elsevier B.V., All rights reserved.
PY - 2009/4
Y1 - 2009/4
N2 - Members of the tumor necrosis factor (TNF)-receptor (R) family may be involved in the tissue remodeling that occurs in the primate corpus luteum (CL) during development and regression. As a first step towards addressing this issue, studies assessed TNF ligand-R expression and regulation in CL collected from monkeys during the early (ECL, Days 3-5), mid (MCL, Days 7-8), mid-late (MLCL, Days 10-11), late (LCL, Days 14-16), and very late (VLCL, menses) luteal phase of the menstrual cycle. CL were also collected after gonadotropin and/or steroid ablation and replacement (with hLH and the progestin R5020) for 3 days at mid-late luteal phase. TNF-a,-p, FAS ligand (FASL), and TNF-R1 mRNA levels were two- to sixfold greater (P < 0.05) at the MLCL or LCL phase as compared to earlier (ECL, MCL). In contrast, TNF-R2 and FAS mRNA levels did not change during the luteal phase. Immunohistochemical staining forTNF-p, TNF-R1, TNF-R2, FAS, and FASL was observed in luteal cells, whereas only TNF-p staining was observed in endothelial cells. Several TNF-R components were influenced by LH and/or steroid ablation; notably, steroid ablation reduced (P< 0.05) luteal TNF-a, but not TNF-p, mRNA levels, which was prevented by progestin treatment. In contrast, steroid ablation increased (P< 0.05) luteal cell immunostaining for FAS and FASL, which was reduced by progestin treatment. Thus, several members of the TNF R-ligand family are expressed in the primate CL in an LH- and/or progestin-dependent manner. Peak expression in the late luteal phase may signify a role for the TNF-R system in death receptor-mediated apoptosis during luteolysis.
AB - Members of the tumor necrosis factor (TNF)-receptor (R) family may be involved in the tissue remodeling that occurs in the primate corpus luteum (CL) during development and regression. As a first step towards addressing this issue, studies assessed TNF ligand-R expression and regulation in CL collected from monkeys during the early (ECL, Days 3-5), mid (MCL, Days 7-8), mid-late (MLCL, Days 10-11), late (LCL, Days 14-16), and very late (VLCL, menses) luteal phase of the menstrual cycle. CL were also collected after gonadotropin and/or steroid ablation and replacement (with hLH and the progestin R5020) for 3 days at mid-late luteal phase. TNF-a,-p, FAS ligand (FASL), and TNF-R1 mRNA levels were two- to sixfold greater (P < 0.05) at the MLCL or LCL phase as compared to earlier (ECL, MCL). In contrast, TNF-R2 and FAS mRNA levels did not change during the luteal phase. Immunohistochemical staining forTNF-p, TNF-R1, TNF-R2, FAS, and FASL was observed in luteal cells, whereas only TNF-p staining was observed in endothelial cells. Several TNF-R components were influenced by LH and/or steroid ablation; notably, steroid ablation reduced (P< 0.05) luteal TNF-a, but not TNF-p, mRNA levels, which was prevented by progestin treatment. In contrast, steroid ablation increased (P< 0.05) luteal cell immunostaining for FAS and FASL, which was reduced by progestin treatment. Thus, several members of the TNF R-ligand family are expressed in the primate CL in an LH- and/or progestin-dependent manner. Peak expression in the late luteal phase may signify a role for the TNF-R system in death receptor-mediated apoptosis during luteolysis.
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U2 - 10.1002/mrd.20970
DO - 10.1002/mrd.20970
M3 - Article
C2 - 18932199
AN - SCOPUS:65449134413
SN - 1040-452X
VL - 76
SP - 367
EP - 378
JO - Molecular Reproduction and Development
JF - Molecular Reproduction and Development
IS - 4
ER -