TY - JOUR
T1 - Expression of insulin-like 3 (INSL3) and differential splicing of its receptor in the ovary of rhesus macaques
AU - Hanna, Carol
AU - Yao, Shan
AU - Patta, Maristela C.
AU - Jensen, Jeffrey T.
AU - Wu, Xuemei
N1 - Funding Information:
The authors thank the following core facilities at Oregon National Primate Research Center: the Assistant Reproductive Technologies (ART) core for assisting in monkey oocyte collection; the Molecular and Cellular Biology (MCB) core for sequencing; and the Imaging and Morphology (IM) core for tissue processing and photographing. Drs John Wade of the University of Melbourne and Richard Ivell of the University of Adelaide provided helpful suggestions for the experiments. The research is supported by a U54 Specialized Cooperative Research Center grant (5U54HD055744) to Drs. Richard Stouffer and J.T.J. from the National Institute of Child Health and Human Development (NICHD). Dr. C.B.H. was a postdoctoral fellow supported by a Reproductive Biology training grant (5T32HD007133) to Dr. Judy Cameron. Dr. M.C. P. was a Fogarty fellow supported by grant TW/HD-00668 to P. Michael Conn.
PY - 2010/12/7
Y1 - 2010/12/7
N2 - Background: Although insulin-like 3 (INSL3) has been identified in the gonad of both sexes in many species, there are only limited reports on the distribution of INSL3 and its receptor, relaxin/insulin-like family peptide receptor 2 (RXFP2), in the primate ovary. Since the hormone-receptor pair is believed to play a role in female reproduction, investigating the transcription of INSL3/RXFP2 genes and the spatiotemporal expression of INSL3 in the nonhuman primate may shed light on the functional aspects of the system in humans.Methods: Database mining, molecular and immunological methods were applied.Results: One single INSL3 transcript and three novel splice variant transcripts of RXFP2 were identified in the ovary of rhesus macaques. While the full-length RXFP2 transcript is barely detectable in granulosa cells during the periovulatory period, INSL3 transcript and protein are highly abundant in theca cells surrounding antral follicles. Moreover, the INSL3 level in follicular fluid is 3-4 times higher than that in female serum which remains low throughout the menstrual cycle.Conclusions: The presence of INSL3 and its receptor in the ovary implies a potential role of the ligand-receptor pair in female reproduction in nonhuman primates. However, the existence of multiple splice variants of RXFP2 indicates a very complex nature of the hormone-receptor system.
AB - Background: Although insulin-like 3 (INSL3) has been identified in the gonad of both sexes in many species, there are only limited reports on the distribution of INSL3 and its receptor, relaxin/insulin-like family peptide receptor 2 (RXFP2), in the primate ovary. Since the hormone-receptor pair is believed to play a role in female reproduction, investigating the transcription of INSL3/RXFP2 genes and the spatiotemporal expression of INSL3 in the nonhuman primate may shed light on the functional aspects of the system in humans.Methods: Database mining, molecular and immunological methods were applied.Results: One single INSL3 transcript and three novel splice variant transcripts of RXFP2 were identified in the ovary of rhesus macaques. While the full-length RXFP2 transcript is barely detectable in granulosa cells during the periovulatory period, INSL3 transcript and protein are highly abundant in theca cells surrounding antral follicles. Moreover, the INSL3 level in follicular fluid is 3-4 times higher than that in female serum which remains low throughout the menstrual cycle.Conclusions: The presence of INSL3 and its receptor in the ovary implies a potential role of the ligand-receptor pair in female reproduction in nonhuman primates. However, the existence of multiple splice variants of RXFP2 indicates a very complex nature of the hormone-receptor system.
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U2 - 10.1186/1477-7827-8-150
DO - 10.1186/1477-7827-8-150
M3 - Article
C2 - 21138583
AN - SCOPUS:78649731235
SN - 1477-7827
VL - 8
JO - Reproductive Biology and Endocrinology
JF - Reproductive Biology and Endocrinology
M1 - 150
ER -