TY - JOUR
T1 - Expression of the ETV6-NTRK3 gene fusion as a primary event in human secretory breast carcinoma
AU - Tognon, Cristina
AU - Knezevich, Stevan R.
AU - Huntsman, David
AU - Roskelley, Calvin D.
AU - Melnyk, Natalya
AU - Mathers, Joan A.
AU - Becker, Laurence
AU - Carneiro, Fatima
AU - MacPherson, Nicol
AU - Horsman, Doug
AU - Poremba, Christopher
AU - Sorensen, Poul H.B.
N1 - Funding Information:
The authors wish to thank Heather Wildgrove, Shelley Moerike, Raihanatou Diallo, and Richard Ma for expert technical assistance. The authors also thank Dr. Martin Gleave for assistance in the mouse studies and Drs. Blake Gilks and Aly Karsan for providing primary breast carcinoma control tissue. This work was supported by funds from the Canadian Institutes for Health Research (to PHBS) and by funds from the Johal Program in Pediatric Oncology Basic and Translational Research at the BC Research Institute for Children's and Women's Health.
PY - 2002/11
Y1 - 2002/11
N2 - We report that human secretory breast carcinoma (SBC), a rare subtype of infiltrating ductal carcinoma, expresses the ETV6-NTRK3 gene fusion previously cloned in pediatric mesenchymal cancers. This gene fusion encodes a chimeric tyrosine kinase with potent transforming activity in fibroblasts. ETV6-NTRK3 expression was confirmed in 12 (92%) of 13 SBC cases, but not in other ductal carcinomas. Retroviral transfer of ETV6-NTRK3 (EN) into murine mammary epithelial cells resulted in transformed cells that readily formed tumors in nude mice. Phenotypically, tumors produced glands and expressed epithelial antigens, confirming that EN transformation is compatible with epithelial differentiation. This represents a recurrent chromosomal rearrangement and expression of a dominantly acting oncogene as a primary event in human breast carcinoma.
AB - We report that human secretory breast carcinoma (SBC), a rare subtype of infiltrating ductal carcinoma, expresses the ETV6-NTRK3 gene fusion previously cloned in pediatric mesenchymal cancers. This gene fusion encodes a chimeric tyrosine kinase with potent transforming activity in fibroblasts. ETV6-NTRK3 expression was confirmed in 12 (92%) of 13 SBC cases, but not in other ductal carcinomas. Retroviral transfer of ETV6-NTRK3 (EN) into murine mammary epithelial cells resulted in transformed cells that readily formed tumors in nude mice. Phenotypically, tumors produced glands and expressed epithelial antigens, confirming that EN transformation is compatible with epithelial differentiation. This represents a recurrent chromosomal rearrangement and expression of a dominantly acting oncogene as a primary event in human breast carcinoma.
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U2 - 10.1016/S1535-6108(02)00180-0
DO - 10.1016/S1535-6108(02)00180-0
M3 - Article
C2 - 12450792
AN - SCOPUS:19044391610
SN - 1535-6108
VL - 2
SP - 367
EP - 376
JO - Cancer Cell
JF - Cancer Cell
IS - 5
ER -