@inbook{dc48f7f70fec4ef793f1baef8bf6c513,
title = "Filtering and Interpreting Large-Scale Experimental Protein–Protein Interaction Data",
abstract = "Rarely acting in isolation, it is invariably the physical associations among proteins that define their biological activity, necessitating the study of the cellular meshwork of protein–protein interactions (PPI) before a full appreciation of gene function can be achieved. The past few years have seen a marked expansion in the both the sheer volume and number of organisms for which high-quality interaction data is available, with high-throughput interaction screening and detection techniques showing consistent improvement both in scale and sensitivity. Although techniques for large-scale PPI mapping are increasingly being applied to new organisms, including human, there is a corresponding need to rigorously evaluate, benchmark, and impartially filter the results. This chapter explores methods for PPI dataset evaluation, including a survey of previous techniques applied by landmark studies in the field and a discussion of promising new experimental approaches. We further outline practical suggestions and useful tools for interpreting newly generated PPI data. As the majority of large-scale experimental data has been generated for the budding yeast S. cerevisiae, most of the techniques and datasets described are from the perspective of this model unicellular eukaryote; however, extensions to other organisms including mammals are mentioned where possible.",
keywords = "Affinity purification, Large-scale assay, Mass spectrometry, Network, Protein–protein interaction, Systems biology, Yeast 2-hybrid",
author = "Gabriel Musso and Andrew Emili and Zhaolei Zhang",
note = "Publisher Copyright: {\textcopyright} 2011, Springer Science+Business Media, LLC.",
year = "2011",
doi = "10.1007/978-1-61779-276-2_14",
language = "English (US)",
isbn = "9781617792755",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "295--309",
booktitle = "Network Biology",
}