First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Kendra A. Hebert; Logan Bateman; Dylan Parker; Jenna Nunziata; Joseph A. Paydarfar; Jennifer Hong; Darcy A. Kerr; George J. Zanazzi; Connor W. Barth; Lei Wang; Summer L. Gibbs; Eric R. Henderson

doi:10.1117/12.2650748

First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Kendra A. Hebert, Logan Bateman, Dylan Parker, Jenna Nunziata, Joseph A. Paydarfar, Jennifer Hong, Darcy A. Kerr, George J. Zanazzi, Connor W. Barth, Lei Wang, Summer L. Gibbs, Eric R. Henderson

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Iatrogenic nerve injury is a common complication across all surgical specialties. Better nerve visualization and identification during surgery will improve outcomes and reduce nerve injuries. The Gibbs Laboratory at Oregon Health and Science University has developed a library of near-infrared, nerve-specific fluorophores to highlight nerves intraoperatively and aid surgeons in nerve identification and visualization; the current lead agent is LGW16-03. Prior to this study, testing of LGW16-03 was restricted to animal models; therefore, it was unknown how LGW16-03 performs in human tissue. To advance LGW16-03 to clinic, we sought to test this current lead agent in ex vivo human tissues from a cohort of patients and determine if the route of administration affects LGW16-03 fluorescence contrast between nerves and adjacent background tissues (muscle and adipose). LGW16-03 was applied to ex vivo human tissue from lower limb amputations via two strategies: (1) systemic administration of the fluorophore using our first-in-kind model for fluorophore testing, and (2) topical application of the fluorophore. Results showed no statistical difference between topical and systemic administration. However, in vivo human validation of these findings is required.

Original language	English (US)
Title of host publication	Molecular-Guided Surgery
Subtitle of host publication	Molecules, Devices, and Applications IX
Editors	Sylvain Gioux, Summer L. Gibbs, Brian W. Pogue
Publisher	SPIE
ISBN (Electronic)	9781510658271
DOIs	https://doi.org/10.1117/12.2650748
State	Published - 2023
Event	Molecular-Guided Surgery: Molecules, Devices, and Applications IX 2023 - San Francisco, United States Duration: Jan 28 2023 → Jan 29 2023

Publication series

Name	Progress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume	12361
ISSN (Print)	1605-7422

Conference

Conference	Molecular-Guided Surgery: Molecules, Devices, and Applications IX 2023
Country/Territory	United States
City	San Francisco
Period	1/28/23 → 1/29/23

Keywords

Fluorescence imaging
ex vivo imaging
fluorophore testing
human testing
lead agent selection
nerve-specific fluorophore

ASJC Scopus subject areas

Electronic, Optical and Magnetic Materials
Atomic and Molecular Physics, and Optics
Biomaterials
Radiology Nuclear Medicine and imaging

Access to Document

10.1117/12.2650748

Cite this

Hebert, K. A., Bateman, L., Parker, D., Nunziata, J., Paydarfar, J. A., Hong, J., Kerr, D. A., Zanazzi, G. J., Barth, C. W., Wang, L., Gibbs, S. L., & Henderson, E. R. (2023). First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues. In S. Gioux, S. L. Gibbs, & B. W. Pogue (Eds.), Molecular-Guided Surgery: Molecules, Devices, and Applications IX Article 123610D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12361). SPIE. https://doi.org/10.1117/12.2650748

First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues. / Hebert, Kendra A.; Bateman, Logan; Parker, Dylan et al.
Molecular-Guided Surgery: Molecules, Devices, and Applications IX. ed. / Sylvain Gioux; Summer L. Gibbs; Brian W. Pogue. SPIE, 2023. 123610D (Progress in Biomedical Optics and Imaging - Proceedings of SPIE; Vol. 12361).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Hebert, KA, Bateman, L, Parker, D, Nunziata, J, Paydarfar, JA, Hong, J, Kerr, DA, Zanazzi, GJ, Barth, CW, Wang, L , Gibbs, SL & Henderson, ER 2023, First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues. in S Gioux, SL Gibbs & BW Pogue (eds), Molecular-Guided Surgery: Molecules, Devices, and Applications IX., 123610D, Progress in Biomedical Optics and Imaging - Proceedings of SPIE, vol. 12361, SPIE, Molecular-Guided Surgery: Molecules, Devices, and Applications IX 2023, San Francisco, United States, 1/28/23. https://doi.org/10.1117/12.2650748

Hebert KA, Bateman L, Parker D, Nunziata J, Paydarfar JA, Hong J et al. First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues. In Gioux S, Gibbs SL, Pogue BW, editors, Molecular-Guided Surgery: Molecules, Devices, and Applications IX. SPIE. 2023. 123610D. (Progress in Biomedical Optics and Imaging - Proceedings of SPIE). doi: 10.1117/12.2650748

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abstract = "Iatrogenic nerve injury is a common complication across all surgical specialties. Better nerve visualization and identification during surgery will improve outcomes and reduce nerve injuries. The Gibbs Laboratory at Oregon Health and Science University has developed a library of near-infrared, nerve-specific fluorophores to highlight nerves intraoperatively and aid surgeons in nerve identification and visualization; the current lead agent is LGW16-03. Prior to this study, testing of LGW16-03 was restricted to animal models; therefore, it was unknown how LGW16-03 performs in human tissue. To advance LGW16-03 to clinic, we sought to test this current lead agent in ex vivo human tissues from a cohort of patients and determine if the route of administration affects LGW16-03 fluorescence contrast between nerves and adjacent background tissues (muscle and adipose). LGW16-03 was applied to ex vivo human tissue from lower limb amputations via two strategies: (1) systemic administration of the fluorophore using our first-in-kind model for fluorophore testing, and (2) topical application of the fluorophore. Results showed no statistical difference between topical and systemic administration. However, in vivo human validation of these findings is required.",

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AB - Iatrogenic nerve injury is a common complication across all surgical specialties. Better nerve visualization and identification during surgery will improve outcomes and reduce nerve injuries. The Gibbs Laboratory at Oregon Health and Science University has developed a library of near-infrared, nerve-specific fluorophores to highlight nerves intraoperatively and aid surgeons in nerve identification and visualization; the current lead agent is LGW16-03. Prior to this study, testing of LGW16-03 was restricted to animal models; therefore, it was unknown how LGW16-03 performs in human tissue. To advance LGW16-03 to clinic, we sought to test this current lead agent in ex vivo human tissues from a cohort of patients and determine if the route of administration affects LGW16-03 fluorescence contrast between nerves and adjacent background tissues (muscle and adipose). LGW16-03 was applied to ex vivo human tissue from lower limb amputations via two strategies: (1) systemic administration of the fluorophore using our first-in-kind model for fluorophore testing, and (2) topical application of the fluorophore. Results showed no statistical difference between topical and systemic administration. However, in vivo human validation of these findings is required.

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First demonstration of a novel nerve-targeting fluorophore in a cohort of ex vivo human tissues

Abstract

Publication series

Conference

Keywords

ASJC Scopus subject areas

Access to Document

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