Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers

Dena Marrinucci; Kelly Bethel; Anand Kolatkar; Madelyn S. Luttgen; Michael Malchiodi; Franziska Baehring; Katharina Voigt; Daniel Lazar; Jorge Nieva; Lyudmila Bazhenova; Andrew H. Ko; W. Michael Korn; Ethan Schram; Michael Coward; Xing Yang; Thomas Metzner; Rachelle Lamy; Meghana Honnatti; Craig Yoshioka; Joshua Kunken; Yelena Petrova; Devin Sok; David Nelson; Peter Kuhn

doi:10.1088/1478-3975/9/1/016003

Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers

Dena Marrinucci, Kelly Bethel, Anand Kolatkar, Madelyn S. Luttgen, Michael Malchiodi, Franziska Baehring, Katharina Voigt, Daniel Lazar, Jorge Nieva, Lyudmila Bazhenova, Andrew H. Ko, W. Michael Korn, Ethan Schram, Michael Coward, Xing Yang, Thomas Metzner, Rachelle Lamy, Meghana Honnatti, Craig Yoshioka, Joshua KunkenYelena Petrova, Devin Sok, David Nelson, Peter Kuhn

Research output: Contribution to journal › Article › peer-review

237 Scopus citations

Abstract

Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL ^-1 of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.

Original language	English (US)
Article number	016003
Journal	Physical Biology
Volume	9
Issue number	1
DOIs	https://doi.org/10.1088/1478-3975/9/1/016003
State	Published - Feb 2012
Externally published	Yes

ASJC Scopus subject areas

Biophysics
Structural Biology
Molecular Biology
Cell Biology

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10.1088/1478-3975/9/1/016003

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Marrinucci, D., Bethel, K., Kolatkar, A., Luttgen, M. S., Malchiodi, M., Baehring, F., Voigt, K., Lazar, D., Nieva, J., Bazhenova, L., Ko, A. H., Korn, W. M., Schram, E., Coward, M., Yang, X., Metzner, T., Lamy, R., Honnatti, M., Yoshioka, C., ... Kuhn, P. (2012). Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers. Physical Biology, 9(1), Article 016003. https://doi.org/10.1088/1478-3975/9/1/016003

Marrinucci, D, Bethel, K, Kolatkar, A, Luttgen, MS, Malchiodi, M, Baehring, F, Voigt, K, Lazar, D, Nieva, J, Bazhenova, L, Ko, AH, Korn, WM, Schram, E, Coward, M, Yang, X, Metzner, T, Lamy, R, Honnatti, M, Yoshioka, C, Kunken, J, Petrova, Y, Sok, D, Nelson, D & Kuhn, P 2012, 'Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers', Physical Biology, vol. 9, no. 1, 016003. https://doi.org/10.1088/1478-3975/9/1/016003

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abstract = "Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL -1 of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.",

author = "Dena Marrinucci and Kelly Bethel and Anand Kolatkar and Luttgen, {Madelyn S.} and Michael Malchiodi and Franziska Baehring and Katharina Voigt and Daniel Lazar and Jorge Nieva and Lyudmila Bazhenova and Ko, {Andrew H.} and Korn, {W. Michael} and Ethan Schram and Michael Coward and Xing Yang and Thomas Metzner and Rachelle Lamy and Meghana Honnatti and Craig Yoshioka and Joshua Kunken and Yelena Petrova and Devin Sok and David Nelson and Peter Kuhn",

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doi = "10.1088/1478-3975/9/1/016003",

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AU - Marrinucci, Dena

AU - Bethel, Kelly

AU - Kolatkar, Anand

AU - Luttgen, Madelyn S.

AU - Malchiodi, Michael

AU - Baehring, Franziska

AU - Voigt, Katharina

AU - Lazar, Daniel

AU - Nieva, Jorge

AU - Bazhenova, Lyudmila

AU - Ko, Andrew H.

AU - Korn, W. Michael

AU - Schram, Ethan

AU - Coward, Michael

AU - Yang, Xing

AU - Metzner, Thomas

AU - Lamy, Rachelle

AU - Honnatti, Meghana

AU - Yoshioka, Craig

AU - Kunken, Joshua

AU - Petrova, Yelena

AU - Sok, Devin

AU - Nelson, David

AU - Kuhn, Peter

PY - 2012/2

Y1 - 2012/2

N2 - Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL -1 of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.

AB - Hematologic spread of carcinoma results in incurable metastasis; yet, the basic characteristics and travel mechanisms of cancer cells in the bloodstream are unknown. We have established a fluid phase biopsy approach that identifies circulating tumor cells (CTCs) without using surface protein-based enrichment and presents them in sufficiently high definition (HD) to satisfy diagnostic pathology image quality requirements. This 'HD-CTC' assay finds >5 HD-CTCs mL -1 of blood in 80% of patients with metastatic prostate cancer (n = 20), in 70% of patients with metastatic breast cancer (n = 30), in 50% of patients with metastatic pancreatic cancer (n = 18), and in 0% of normal controls (n = 15). Additionally, it finds HD-CTC clusters ranging from 2 HD-CTCs to greater than 30 HD-CTCs in the majority of these cancer patients. This initial validation of an enrichment-free assay demonstrates our ability to identify significant numbers of HD-CTCs in a majority of patients with prostate, breast and pancreatic cancers.

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Fluid biopsy in patients with metastatic prostate, pancreatic and breast cancers

Abstract

ASJC Scopus subject areas

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