Fluorescence visualization of newly synthesized proteins in mammalian cells

Kimberly E. Beatty; Julie C. Liu; Fang Xie; Daniela C. Dieterich; Erin M. Schuman; Qian Wang; David A. Tirrell

doi:10.1002/anie.200602114

Fluorescence visualization of newly synthesized proteins in mammalian cells

Kimberly E. Beatty, Julie C. Liu, Fang Xie, Daniela C. Dieterich, Erin M. Schuman, Qian Wang, David A. Tirrell

Research output: Contribution to journal › Article › peer-review

241 Scopus citations

Abstract

(Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.

Original language	English (US)
Pages (from-to)	7364-7367
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	45
Issue number	44
DOIs	https://doi.org/10.1002/anie.200602114
State	Published - Nov 13 2006
Externally published	Yes

Keywords

Alkynes
Azides
Fluorescent probes
Protein modifications
Proteomics

ASJC Scopus subject areas

Catalysis
General Chemistry

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10.1002/anie.200602114

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title = "Fluorescence visualization of newly synthesized proteins in mammalian cells",

abstract = "(Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.",

keywords = "Alkynes, Azides, Fluorescent probes, Protein modifications, Proteomics",

author = "Beatty, {Kimberly E.} and Liu, {Julie C.} and Fang Xie and Dieterich, {Daniela C.} and Schuman, {Erin M.} and Qian Wang and Tirrell, {David A.}",

year = "2006",

month = nov,

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T1 - Fluorescence visualization of newly synthesized proteins in mammalian cells

AU - Beatty, Kimberly E.

AU - Liu, Julie C.

AU - Xie, Fang

AU - Dieterich, Daniela C.

AU - Schuman, Erin M.

AU - Wang, Qian

AU - Tirrell, David A.

PY - 2006/11/13

Y1 - 2006/11/13

N2 - (Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.

AB - (Figure Presented) Playing tag: Noncanonical amino acid tagging enables the selective fluorescent visualization of newly synthesized proteins in mammalian cells (see the picture). Susceptibility to tagging is determined by the spatial and temporal character of the protein synthesis, thus providing a complement to methods which identify relevant members of the proteome.

KW - Alkynes

KW - Azides

KW - Fluorescent probes

KW - Protein modifications

KW - Proteomics

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U2 - 10.1002/anie.200602114

DO - 10.1002/anie.200602114

M3 - Article

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JF - Angewandte Chemie - International Edition

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ER -

Fluorescence visualization of newly synthesized proteins in mammalian cells

Abstract

Keywords

ASJC Scopus subject areas

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