Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Eltot R. Spindel; Michael Lakher; Wurtman Richard J.

doi:10.1016/0006-8993(81)90136-0

Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Eltot R. Spindel, Michael Lakher, Wurtman Richard J.

Research output: Contribution to journal › Article › peer-review

11 Scopus citations

Abstract

Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

Original language	English (US)
Pages (from-to)	343-350
Number of pages	8
Journal	Brain research
Volume	216
Issue number	2
DOIs	https://doi.org/10.1016/0006-8993(81)90136-0
State	Published - Jul 20 1981
Externally published	Yes

Keywords

TRH
TRH metabolism
TRH turnover
deamido-TRH
neuropeptide metabolism

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/0006-8993(81)90136-0

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@article{be90710c2c2a485a99724bb539cc7739,

title = "Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH",

abstract = "Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.",

keywords = "TRH, TRH metabolism, TRH turnover, deamido-TRH, neuropeptide metabolism",

author = "Spindel, {Eltot R.} and Michael Lakher and {Richard J.}, Wurtman",

note = "Funding Information: These studies were supported in part by grants from the National Institutes of Health (AA-14288) and the Center for Brain Sciences and Metabolism, Charitable Trust.",

year = "1981",

month = jul,

day = "20",

doi = "10.1016/0006-8993(81)90136-0",

language = "English (US)",

volume = "216",

pages = "343--350",

journal = "Brain research",

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publisher = "Elsevier",

number = "2",

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T1 - Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

AU - Spindel, Eltot R.

AU - Lakher, Michael

AU - Richard J., Wurtman

N1 - Funding Information: These studies were supported in part by grants from the National Institutes of Health (AA-14288) and the Center for Brain Sciences and Metabolism, Charitable Trust.

PY - 1981/7/20

Y1 - 1981/7/20

N2 - Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

AB - Thyrotropin-releasing hormone (TRH) injected into the lateral ventricle of the rat's brain was rapidly metabolized to deamido-TRH (DA-TRH). Brain levels of TRH decreased with a half-life of 7 min, while the DA-TRH formed from it disappeared with a half-life of 2.5 min. To prevent the post-mortem degradation of DA-TRH it was necessary to sacrifice rats by directing focused microwave irradiation towards the brain. The short half-lives determined for TRH and DA-TRH in vivo were much shorter than those obtained using in vitro techniques. The in vivo formation and accumulation of DA-TRH was inhibited by bacitracin and unaffected by probenicid.

KW - TRH

KW - TRH metabolism

KW - TRH turnover

KW - deamido-TRH

KW - neuropeptide metabolism

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DO - 10.1016/0006-8993(81)90136-0

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VL - 216

SP - 343

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JO - Brain research

JF - Brain research

IS - 2

ER -

Formation and degradation of deamido-TRH (pyroglutamyl-histidyl-proline) in rat brain after intraventricular injection of TRH

Abstract

Keywords

ASJC Scopus subject areas

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