Beta and gamma endorphin-like peptides were measured by radioimmunoassay in whole pituitary. Boiling of acetic acid extracts prior to tissue disruption increased the concentration of both βE- and γE-like peptides. The γE-like immunoreactivity from the neurointermediate lobe of the pituitary co-eluted with synthetic γE upon gel permeation chromatography. Immunoreactivity for βE-like and γE-like peptides in the intermediate lobe of the pituitary was also shown by immunoperoxidase staining. The results suggest that γE-like peptides are present primarily in the pars intermedia in vivo and do not arise as artifacts of acid extraction of pituitary tissue.
ASJC Scopus subject areas
- Cellular and Molecular Neuroscience