@article{b1a08983cf0b4790b8412da416871081,
title = "Gbx2 Identifies Two Amacrine Cell Subtypes with Distinct Molecular, Morphological, and Physiological Properties",
abstract = "Our understanding of nervous system function is limited by our ability to identify and manipulate neuronal subtypes within intact circuits. We show that the Gbx2CreERT2-IRES-EGFP mouse line labels two amacrine cell (AC) subtypes in the mouse retina that have distinct morphological, physiological, and molecular properties. Using a combination of RNA-seq, genetic labeling, and patch clamp recordings, we show that one subtype is GABAergic that receives excitatory input from On bipolar cells. The other population is a non-GABAergic, non-glycinergic (nGnG) AC subtype that lacks the expression of standard neurotransmitter markers. Gbx2+ nGnG ACs have smaller, asymmetric dendritic arbors that receive excitatory input from both On and Off bipolar cells. Gbx2+ nGnG ACs also exhibit spatially restricted tracer coupling to bipolar cells (BCs) through gap junctions. This study identifies a genetic tool for investigating the two distinct AC subtypes, and it provides a model for studying synaptic communication and visual circuit function.",
keywords = "amacrine, electrical synapses, neural circuit, neuronal identity, retina, vision",
author = "Kerstein, {Patrick C.} and Joseph Leffler and Benjamin Sivyer and Taylor, {W. Rowland} and Wright, {Kevin M.}",
note = "Funding Information: We would like to thank the members of the Wright, Taylor, and Sivyer laboratories for their assistance and discussion throughout the course of this study. We also thank Dr. Kelly Monk and Dr. Martin Riccomagno for their comments on the manuscript. This work was supported by NIH grant R01 NS091027 , a Whitehall Foundation research grant , and an OHSU University Shared Resources Grant , to K.M.W.; NIH grant F32 EY029974 , the Collins Medical Trust , and the Knights Templar Eye Foundation , to P.C.K.; NIH grant T32 NS007466 , to J.L.; NIH grants R01 EY022070 and P30 EY003176 , to W.R.T.; and NIH grant P30 EY010572 and unrestricted departmental funding from Research to Prevent Blindness (New York, NY) and support from the donors of National Glaucoma Research, a program of BrightFocus Foundation , to B.S. Confocal microscopy and analysis was performed in the OHSU Advance Light Microscopy Core supported by NIH grant P30 NS061800 . Cell sorting was performed in the OHSU Flow Cytometry Shared Resource, and the core is supported by the Knight NCI Cancer Center Support Grant . Short-read sequencing assays were performed by Dr. Amy Carlos and Dr. Robert Searles of the OHSU Massively Parallel Sequencing Shared Resource. The authors acknowledge the support of Dr. Suzanne Fei and Dr. Lina Gao of the Oregon National Primate Research Center Bioinformatics and Biostatistics Core, which is funded in part by NIH grant OD P51 OD011092 . Publisher Copyright: {\textcopyright} 2020 The Authors",
year = "2020",
month = nov,
day = "17",
doi = "10.1016/j.celrep.2020.108382",
language = "English (US)",
volume = "33",
journal = "Cell Reports",
issn = "2211-1247",
publisher = "Cell Press",
number = "7",
}