Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D. Lara; Sissy E. Wamaitha; Auriana Arabpour; Jon D. Hennebold; Amander T. Clark; Enrique Sosa

doi:10.1016/j.scr.2023.103241

Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

Mary Jasmine D. Lara, Sissy E. Wamaitha, Auriana Arabpour, Jon D. Hennebold, Amander T. Clark, Enrique Sosa

Oregon National Primate Research Center

Research output: Contribution to journal › Article › peer-review

Abstract

We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.

Original language	English (US)
Article number	103241
Journal	Stem Cell Research
Volume	73
DOIs	https://doi.org/10.1016/j.scr.2023.103241
State	Published - Dec 2023

ASJC Scopus subject areas

Developmental Biology
Cell Biology

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10.1016/j.scr.2023.103241

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title = "Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions",

abstract = "We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.",

author = "Lara, {Mary Jasmine D.} and Wamaitha, {Sissy E.} and Auriana Arabpour and Hennebold, {Jon D.} and Clark, {Amander T.} and Enrique Sosa",

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doi = "10.1016/j.scr.2023.103241",

language = "English (US)",

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AU - Lara, Mary Jasmine D.

AU - Wamaitha, Sissy E.

AU - Arabpour, Auriana

AU - Hennebold, Jon D.

AU - Clark, Amander T.

AU - Sosa, Enrique

PY - 2023/12

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AB - We generated and characterized a rhesus macaque induced pluripotent stem cell (iPSC) line using induced reprogramming of fibroblasts isolated from a rhesus macaque fetus. The fibroblasts were expanded and then reprogrammed using non-integrating Sendai virus technology. This line is available as riPSC05. The authenticity of riPSC05 was confirmed through the expression of pluripotent and self-renewal markers, in vitro-directed differentiation towards three germ layers (ectoderm, mesoderm, and endoderm), karyotyping, and STR analysis.

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Generation of a rhesus macaque induced pluripotent stem cell line (riPSC05) under feeder-free conditions

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