Genetically encoded FRET probe for PKC activity based on pleckstrin

Andreas Schleifenbaum; Gunter Stier; Alexander Gasch; Michael Sattler; Garsten Schultz

doi:10.1021/ja0460155

Genetically encoded FRET probe for PKC activity based on pleckstrin

Andreas Schleifenbaum, Gunter Stier, Alexander Gasch, Michael Sattler, Garsten Schultz

Research output: Contribution to journal › Article › peer-review

62 Scopus citations

Abstract

We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.

Original language	English (US)
Pages (from-to)	11786-11787
Number of pages	2
Journal	Journal of the American Chemical Society
Volume	126
Issue number	38
DOIs	https://doi.org/10.1021/ja0460155
State	Published - Sep 29 2004
Externally published	Yes

ASJC Scopus subject areas

Catalysis
Chemistry(all)
Biochemistry
Colloid and Surface Chemistry

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abstract = "We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.",

author = "Andreas Schleifenbaum and Gunter Stier and Alexander Gasch and Michael Sattler and Garsten Schultz",

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AU - Schleifenbaum, Andreas

AU - Stier, Gunter

AU - Gasch, Alexander

AU - Sattler, Michael

AU - Schultz, Garsten

PY - 2004/9/29

Y1 - 2004/9/29

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AB - We developed a probe for investigating protein kinase C (PKC) activity in living cells. The probe is based on a fragment of pleckstrin enclosed by two FRET-capable fluorophores. PKC activity modulation was reliably followed by FRET change in vitro and in vivo. The probe responds quickly to PKC activation by phorbol ester. FRET changes were reversible when PKC inhibitors were administered. Stimulation of cellular signaling pathways using histamine or bradykinin triggered PKC in a physiologically relevant way.

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JF - Journal of the American Chemical Society

IS - 38

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Genetically encoded FRET probe for PKC activity based on pleckstrin

Abstract

ASJC Scopus subject areas

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