TY - JOUR
T1 - Genome-scale genetic manipulation methods for exploring bacterial molecular biology
AU - Gagarinova, Alla
AU - Emili, Andrew
PY - 2012/6
Y1 - 2012/6
N2 - Bacteria are diverse and abundant, playing key roles in human health and disease, the environment, and biotechnology. Despite progress in genome sequencing and bioengineering, much remains unknown about the functional organization of prokaryotes. For instance, roughly a third of the protein-coding genes of the best-studied model bacterium, Escherichia coli, currently lack experimental annotations. Systems-level experimental approaches for investigating the functional associations of bacterial genes and genetic structures are essential for defining the fundamental molecular biology of microbes, preventing the spread of antibacterial resistance in the clinic, and driving the development of future biotechnological applications. This review highlights recently introduced large-scale genetic manipulation and screening procedures for the systematic exploration of bacterial gene functions, molecular relationships, and the global organization of bacteria at the gene, pathway, and genome levels.
AB - Bacteria are diverse and abundant, playing key roles in human health and disease, the environment, and biotechnology. Despite progress in genome sequencing and bioengineering, much remains unknown about the functional organization of prokaryotes. For instance, roughly a third of the protein-coding genes of the best-studied model bacterium, Escherichia coli, currently lack experimental annotations. Systems-level experimental approaches for investigating the functional associations of bacterial genes and genetic structures are essential for defining the fundamental molecular biology of microbes, preventing the spread of antibacterial resistance in the clinic, and driving the development of future biotechnological applications. This review highlights recently introduced large-scale genetic manipulation and screening procedures for the systematic exploration of bacterial gene functions, molecular relationships, and the global organization of bacteria at the gene, pathway, and genome levels.
UR - http://www.scopus.com/inward/record.url?scp=84861125187&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861125187&partnerID=8YFLogxK
U2 - 10.1039/c2mb25040c
DO - 10.1039/c2mb25040c
M3 - Review article
C2 - 22517266
AN - SCOPUS:84861125187
SN - 1742-206X
VL - 8
SP - 1626
EP - 1638
JO - Molecular BioSystems
JF - Molecular BioSystems
IS - 6
ER -