TY - JOUR
T1 - Hexokinase redistribution in vivo
AU - Laursen, S. E.
AU - Belknap, J. K.
AU - Sampson, K. E.
AU - Knull, H. R.
N1 - Funding Information:
The donation of the WSR and WSP mice by Dr. John Crabbe is greatly appreciated. The authors express special thanks to Marlys Kennedy, Mary Bohlman and Donna Carr for their kind help with manuscript preparation. This research was supported by NIAAA grant AA06243 and NIH grant NS17711. Additional support was provided from a VA grant awarded to J.K.B.
PY - 1990/4/23
Y1 - 1990/4/23
N2 - Heterogenous stock mice in addition to mice selectively bred to maximally differ in their severity of alcohol withdrawal seizures (withdrawal seizure-resistant (WSR) and withdrawal seizure-prone (WSP)) were used to provide evidence in favor of the importance of the rapidly changing distribution of brain hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1) (HK). An ischemic response at 15, 30, 60 and 120 s after killing showed a decreasing cerebellar cytosolic HK concentration of 31%, 15%, 14% and 10% while the cerebral concentrations were 23%, 13% and 14%, respectivetively. WSR and WSP mice given an acute i.p. dose 4 g/kg of alcohol showed opposite HK responses. Cytosolic HK in WSR mice decreased 18.5%, while WSP mice showed an increased of 20.3% over paired saline-injected controls. When ischemia was allowed to proceed in WSP mice following an in vivo alcohol treatment, cytosolic HK decreased in parallel to mice not given alcohol. These data suggest that alcohol can cause an HK redistribution in vivo which could play a role in the difering sensitivities of WSR and WSP mice to alcohol related seizures.
AB - Heterogenous stock mice in addition to mice selectively bred to maximally differ in their severity of alcohol withdrawal seizures (withdrawal seizure-resistant (WSR) and withdrawal seizure-prone (WSP)) were used to provide evidence in favor of the importance of the rapidly changing distribution of brain hexokinase (ATP: D-hexose 6-phosphotransferase, EC 2.7.1.1) (HK). An ischemic response at 15, 30, 60 and 120 s after killing showed a decreasing cerebellar cytosolic HK concentration of 31%, 15%, 14% and 10% while the cerebral concentrations were 23%, 13% and 14%, respectivetively. WSR and WSP mice given an acute i.p. dose 4 g/kg of alcohol showed opposite HK responses. Cytosolic HK in WSR mice decreased 18.5%, while WSP mice showed an increased of 20.3% over paired saline-injected controls. When ischemia was allowed to proceed in WSP mice following an in vivo alcohol treatment, cytosolic HK decreased in parallel to mice not given alcohol. These data suggest that alcohol can cause an HK redistribution in vivo which could play a role in the difering sensitivities of WSR and WSP mice to alcohol related seizures.
KW - Alcohol
KW - Alcohol withdrawal seizure
KW - Hexokinase
KW - Ischemia
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U2 - 10.1016/0304-4165(90)90162-P
DO - 10.1016/0304-4165(90)90162-P
M3 - Article
C2 - 2328257
AN - SCOPUS:0025213925
SN - 0304-4165
VL - 1034
SP - 118
EP - 121
JO - BBA - General Subjects
JF - BBA - General Subjects
IS - 1
ER -