TY - JOUR
T1 - High-Throughput Detection of Glutathione S-Transferase Polymorphic Alleles in a Pediatric Cancer Population
AU - Barnette, Phillip
AU - Scholl, Rebecca
AU - Blandford, Mary
AU - Ballard, Linda
AU - Tsodikov, Alexander
AU - Magee, Jalene
AU - Williams, Susana
AU - Robertson, Margaret
AU - Ali-Osman, Francis
AU - Lemons, Richard
AU - Keller, Charles
PY - 2004/2
Y1 - 2004/2
N2 - Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. We report a low cost, highly reproducible and specific PCR-based high-throughput assay for genotyping different GSTs designed for use in large clinical trials. In comparison to an alternative genotyping method (single nucleotide extension), the sensitivity and specificity of the high throughput assay was shown to be 92 and 97%, respectively, depending on the source of genomic DNA. Using the high-throughput assay, we demonstrate by multivariate analysis an increased risk of acute lymphoblastic leukemia, glial brain tumors, and osteosarcoma for patients carrying nonnull alleles of GSTM1 and/or GSTT1.
AB - Polymorphisms of glutathione S-transferase (GST) enzymes have been correlated with altered risk of several cancers, as well as altered response and toxicity from cancer chemotherapy. We report a low cost, highly reproducible and specific PCR-based high-throughput assay for genotyping different GSTs designed for use in large clinical trials. In comparison to an alternative genotyping method (single nucleotide extension), the sensitivity and specificity of the high throughput assay was shown to be 92 and 97%, respectively, depending on the source of genomic DNA. Using the high-throughput assay, we demonstrate by multivariate analysis an increased risk of acute lymphoblastic leukemia, glial brain tumors, and osteosarcoma for patients carrying nonnull alleles of GSTM1 and/or GSTT1.
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U2 - 10.1158/1055-9965.EPI-03-0178
DO - 10.1158/1055-9965.EPI-03-0178
M3 - Article
C2 - 14973099
AN - SCOPUS:10744233323
SN - 1055-9965
VL - 13
SP - 304
EP - 313
JO - Cancer Epidemiology Biomarkers and Prevention
JF - Cancer Epidemiology Biomarkers and Prevention
IS - 2
ER -