Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Ov Daniel Slayden; Francis Kathryn E. Friason; Kise Rosen Bond; Emily Cathleen Mishler

doi:10.1111/jmp.12357

Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

Ov Daniel Slayden, Francis Kathryn E. Friason, Kise Rosen Bond, Emily Cathleen Mishler

Oregon National Primate Research Center

Research output: Contribution to journal › Article › peer-review

7 Scopus citations

Abstract

Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E₂), E₂ plus progesterone (P), and E₂ plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E₂ and reduced after treatment with E₂ + P (P <.05). LNG also reduced OVGP1 expression (P <.05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

Original language	English (US)
Pages (from-to)	362-370
Number of pages	9
Journal	Journal of medical primatology
Volume	47
Issue number	6
DOIs	https://doi.org/10.1111/jmp.12357
State	Published - Dec 2018

Keywords

cervix
cytobrush
hormonal regulation
oviductal glycoprotein
progestogen

ASJC Scopus subject areas

Animal Science and Zoology
veterinary(all)

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10.1111/jmp.12357

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title = "Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix",

abstract = "Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P <.05). LNG also reduced OVGP1 expression (P <.05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.",

keywords = "cervix, cytobrush, hormonal regulation, oviductal glycoprotein, progestogen",

author = "Slayden, {Ov Daniel} and Friason, {Francis Kathryn E.} and Bond, {Kise Rosen} and Mishler, {Emily Cathleen}",

note = "Funding Information: The authors thank the Division of Comparative Medicine at the Oregon National Primate Research Center for assistance with animal studies; Shan Yao for assistance with Western blot, Fangzhou Luo for immunohistochemistry and Corinne Wilcox for help with RT-qPCR analysis. This work was supported by NIH grants U54 HD055744; NIH P51OD011092. Publisher Copyright: {\textcopyright} 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd",

year = "2018",

month = dec,

doi = "10.1111/jmp.12357",

language = "English (US)",

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AU - Slayden, Ov Daniel

AU - Friason, Francis Kathryn E.

AU - Bond, Kise Rosen

AU - Mishler, Emily Cathleen

N1 - Funding Information: The authors thank the Division of Comparative Medicine at the Oregon National Primate Research Center for assistance with animal studies; Shan Yao for assistance with Western blot, Fangzhou Luo for immunohistochemistry and Corinne Wilcox for help with RT-qPCR analysis. This work was supported by NIH grants U54 HD055744; NIH P51OD011092. Publisher Copyright: © 2018 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd

PY - 2018/12

Y1 - 2018/12

N2 - Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P <.05). LNG also reduced OVGP1 expression (P <.05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

AB - Background: Macaques are outstanding animal models for the development of new contraceptives. In women, progestin-only contraceptives often fail to block ovulation and are believed to act by altering cervix physiology. Herein, we assessed oviductal glycoprotein 1 (OVGP1) in the macaque cervix as a marker for progestogen action. Materials: Rhesus macaques were treated with estradiol (E2), E2 plus progesterone (P), and E2 plus levonorgestrel (LNG), a contraceptive progestin. Samples consisted of archived blocks of midcervix mucosa (epithelium and lamina propria) and fresh epithelial cells collected non-invasively by cytobrush. OVGP1 was assayed by quantitative real-time PCR and localized by immunocytochemistry. Results: OVGP1 transcript was maximal after E2 and reduced after treatment with E2 + P (P <.05). LNG also reduced OVGP1 expression (P <.05). OVGP1-specific staining localized to epithelial cells, and transcript was quantifiable in cytobrush collected samples. Conclusions: OVGP1 expression in cytobrush samples of macaque cervix provides a non-invasive indicator of contraceptive progestin action.

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Hormonal regulation of oviductal glycoprotein 1 (OVGP1; MUC9) in the rhesus macaque cervix

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