Abstract
The production of both protein and steroid hormones was studied utilizing somatic cell hybrids formed with human choriocarcinoma cells. The human JEG-3 cell line produced the species and organ-specific hormone human chorionic gonadotropin (hCG), the steroid hormone progesterone, and converted 19-carbon steroids to estrogens. Hybrids formed with human VA-2 cells, mouse Cl 1D cells and mouse 3T3-4EF cells had detectable hCG synthesis in 20 of 41 total clones. There was no detectable progesterone or 19-carbon aromatization to estrogens in any hybrids. These data demonstrate that the differentiated function of human protein hormone production can be retained in inter- and intra-specific somatic cell hybrids. These results also suggest that protein hormone production can occur independently of steroid production in these cells of placental origin.
Original language | English (US) |
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Pages (from-to) | 303-310 |
Number of pages | 8 |
Journal | Experimental Cell Research |
Volume | 103 |
Issue number | 2 |
DOIs | |
State | Published - Dec 1976 |
Externally published | Yes |
ASJC Scopus subject areas
- Cell Biology