Human monocytes and macrophages: Interaction with antigen and lymphocytes

J. M. Hanifin, M. J. Cline

Research output: Contribution to journalArticlepeer-review

56 Scopus citations


PPD-sensitized monocytes and macrophages from tuberculin-positive subjects are both capable of inducing blastogenic transformation of autologous lymphocytes. Incorporation of thymidine-3H and morphological transformation were always greater in lymphocyte cultures containing macrophages than in those containing monocytes. More lymphocytes entered the first detectable S phase in cultures containing macrophages. Lymphocyte DNA synthesis occurred as early as 40 hr of culture and always in cells in contact with mononuclear phagocytes. By 120-144 hr, many transformed lymphocytes were free in suspension; at the same time, the "immunological cluster" had increased greatly in size and contained transformed and untransformed lymphocytes. The greater effectiveness of macrophages at induction of lymphocyte transformation may be related to the efficiency of this cell type at trapping antigen and its effectiveness at making contact with and binding lymphocytes.

Original languageEnglish (US)
Pages (from-to)97-105
Number of pages9
JournalJournal of Cell Biology
Issue number1
StatePublished - Jul 1 1970
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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