Identification of enzyme-converted peptide products from single cells using capillary electrophoresis and liquid chromatography-mass spectrometry

Robert B. Brown, Johannes A. Hewel, Andrew Emili, Julie Audet

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

Single-cell analysis using chemical methods, otherwise known as chemical cytometry, promises to provide significant leaps in understanding signaling processes which result in cellular behavior. Sensitive methods for chemical cytometry such as capillary electrophoresis can detect and quantify multiple targets; however, conclusive identification of detected analytes is required for useful data to be obtained. Here, we demonstrate a method for determining the identity of enzyme-converted peptide products from single cells using a combination of capillary electrophoresis and liquid chromatography-mass spectrometry (LC-MS).

Original languageEnglish (US)
Title of host publicationSingle-Cell Analysis
Subtitle of host publicationMethods and Protocols
EditorsSara Lindstrom, Helene Andersson-Svahn
Pages17-28
Number of pages12
DOIs
StatePublished - 2012
Externally publishedYes

Publication series

NameMethods in Molecular Biology
Volume853
ISSN (Print)1064-3745

Keywords

  • Capillary electrophoresis
  • Chemical cytometry
  • Enzyme activity
  • Fluorescent peptide
  • Mass spectrometry
  • Single cell

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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