IFN-I and IL-22 mediate protective effects of intestinal viral infection

Jessica A. Neil, Yu Matsuzawa-Ishimoto, Elisabeth Kernbauer-Hölzl, Samantha L. Schuster, Stela Sota, Mericien Venzon, Simone Dallari, Antonio Galvao Neto, Ashley Hine, David Hudesman, P’ng Loke, Timothy J. Nice, Ken Cadwell

Research output: Contribution to journalArticlepeer-review

52 Scopus citations


Products derived from bacterial members of the gut microbiota evoke immune signalling pathways of the host that promote immunity and barrier function in the intestine. How immune reactions to enteric viruses support intestinal homeostasis is unknown. We recently demonstrated that infection by murine norovirus (MNV) reverses intestinal abnormalities following depletion of bacteria, indicating that an intestinal animal virus can provide cues to the host that are typically attributed to the microbiota. Here, we elucidate mechanisms by which MNV evokes protective responses from the host. We identify an important role for the viral protein NS1/2 in establishing local replication and a type I interferon (IFN-I) response in the colon. We further show that IFN-I acts on intestinal epithelial cells to increase the proportion of CCR2-dependent macrophages and interleukin (IL)-22-producing innate lymphoid cells, which in turn promote pSTAT3 signalling in intestinal epithelial cells and protection from intestinal injury. In addition, we demonstrate that MNV provides a striking IL-22-dependent protection against early-life lethal infection by Citrobacter rodentium. These findings demonstrate novel ways in which a viral member of the microbiota fortifies the intestinal barrier during chemical injury and infectious challenges.

Original languageEnglish (US)
Pages (from-to)1737-1749
Number of pages13
JournalNature Microbiology
Issue number10
StatePublished - Oct 1 2019

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology
  • Genetics
  • Microbiology (medical)
  • Cell Biology


Dive into the research topics of 'IFN-I and IL-22 mediate protective effects of intestinal viral infection'. Together they form a unique fingerprint.

Cite this