In situ analyses of genome instability in breast cancer

Koei Chin, Carlos Ortiz De Solorzano, David Knowles, Arthur Jones, William Chou, Enrique Garcia Rodriguez, Wen Lin Kuo, Britt Marie Ljung, Karen Chew, Kenneth Myambol, Monica Miranda, Sheryl Krig, James Garbe, Martha Stampfer, Paul Yaswen, Joe W. Gray, Stephen J. Lockett

Research output: Contribution to journalArticlepeer-review

316 Scopus citations


Transition through telomere crisis is thought to be a crucial event in the development of most breast carcinomas. Our goal in this study was to determine where this occurs in the context of histologically defined breast cancer progression. To this end, we assessed genome instability (using fluorescence in situ hybridization) and other features associated with telomere crisis in normal ductal epithelium, usual ductal hyperplasia, ductal carcinoma in situ and invasive cancer. We modeled this process in vitro by measuring these same features in human mammary epithelial cell cultures during ZNF217-mediated transition through telomere crisis and immortalization. Taken together, the data suggest that transition through telomere crisis and immortalization in breast cancer occurs during progression from usual ductal hyperplasia to ductal carcinoma in situ.

Original languageEnglish (US)
Pages (from-to)984-988
Number of pages5
JournalNature genetics
Issue number9
StatePublished - Sep 2004
Externally publishedYes

ASJC Scopus subject areas

  • Genetics


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