In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer

Arunporn Itharat; Peter J. Houghton; E. Eno-Amooquaye; P. J. Burke; Julia H. Sampson; Amala Raman

doi:10.1016/j.jep.2003.09.014

In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer

Arunporn Itharat, Peter J. Houghton, E. Eno-Amooquaye, P. J. Burke, Julia H. Sampson, Amala Raman

Research output: Contribution to journal › Article › peer-review

215 Scopus citations

Abstract

The SRB assay was used to test cytotoxicity against three human cancer cell lines and one normal cell line of 11 Thai medicinal plant species used by traditional doctors in treating cancer patients. The extraction procedures used were similar to those practised by Thai traditional doctors (ethanolic and water extracts). Extracts were tested against the human large cell lung carcinoma cell line COR-L23, the human breast adenocarcinoma cell line MCF-7 and human colon adenocarcinoma cell line LS-174T and normal human keratinocytes SVK-14. The results showed that three plants; Dioscorea membranacea Pierre ex Prain & Burkill, Dioscorea birmanica Prain & Burkill (Dioscoreaceae) and Siphonodon celastrineus Griff. (Celastraceae), exhibited high cytotoxic activity showing a certain degree of selectivity against the different cell types.

Original language	English (US)
Pages (from-to)	33-38
Number of pages	6
Journal	Journal of Ethnopharmacology
Volume	90
Issue number	1
DOIs	https://doi.org/10.1016/j.jep.2003.09.014
State	Published - Jan 2004
Externally published	Yes

Keywords

Cytotoxicity test
Dioscorea
SRB assay
Siphonodon
Thai anticancer plants

ASJC Scopus subject areas

Pharmacology
Drug Discovery

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10.1016/j.jep.2003.09.014

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title = "In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer",

abstract = "The SRB assay was used to test cytotoxicity against three human cancer cell lines and one normal cell line of 11 Thai medicinal plant species used by traditional doctors in treating cancer patients. The extraction procedures used were similar to those practised by Thai traditional doctors (ethanolic and water extracts). Extracts were tested against the human large cell lung carcinoma cell line COR-L23, the human breast adenocarcinoma cell line MCF-7 and human colon adenocarcinoma cell line LS-174T and normal human keratinocytes SVK-14. The results showed that three plants; Dioscorea membranacea Pierre ex Prain & Burkill, Dioscorea birmanica Prain & Burkill (Dioscoreaceae) and Siphonodon celastrineus Griff. (Celastraceae), exhibited high cytotoxic activity showing a certain degree of selectivity against the different cell types.",

keywords = "Cytotoxicity test, Dioscorea, SRB assay, Siphonodon, Thai anticancer plants",

author = "Arunporn Itharat and Houghton, {Peter J.} and E. Eno-Amooquaye and Burke, {P. J.} and Sampson, {Julia H.} and Amala Raman",

note = "Funding Information: The authors wish to thank three Thai traditional doctors, Dr. Prakob Ubonkhao, Dr. Jon Phensuk and Dr. Pinkao Khongnuan for assistance in the collection of plants. Dr. Tippun Sdakorn, a botanist from Department of Forestry, Ministry of Agriculture, helped identify the plants. We are grateful to the Thai Traditional Institute for financial support for studying the folk wisdom of Thai Traditional doctors in the treatment of cancer patients. Special thanks is due to the British Technical Co-operation Training Scholarship for supporting the training course for Mrs. Itharat in {\textquoteleft}Cytotoxicity testing from Plant Extracts{\textquoteright}. ",

year = "2004",

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doi = "10.1016/j.jep.2003.09.014",

language = "English (US)",

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AU - Itharat, Arunporn

AU - Houghton, Peter J.

AU - Eno-Amooquaye, E.

AU - Burke, P. J.

AU - Sampson, Julia H.

AU - Raman, Amala

N1 - Funding Information: The authors wish to thank three Thai traditional doctors, Dr. Prakob Ubonkhao, Dr. Jon Phensuk and Dr. Pinkao Khongnuan for assistance in the collection of plants. Dr. Tippun Sdakorn, a botanist from Department of Forestry, Ministry of Agriculture, helped identify the plants. We are grateful to the Thai Traditional Institute for financial support for studying the folk wisdom of Thai Traditional doctors in the treatment of cancer patients. Special thanks is due to the British Technical Co-operation Training Scholarship for supporting the training course for Mrs. Itharat in ‘Cytotoxicity testing from Plant Extracts’.

PY - 2004/1

Y1 - 2004/1

N2 - The SRB assay was used to test cytotoxicity against three human cancer cell lines and one normal cell line of 11 Thai medicinal plant species used by traditional doctors in treating cancer patients. The extraction procedures used were similar to those practised by Thai traditional doctors (ethanolic and water extracts). Extracts were tested against the human large cell lung carcinoma cell line COR-L23, the human breast adenocarcinoma cell line MCF-7 and human colon adenocarcinoma cell line LS-174T and normal human keratinocytes SVK-14. The results showed that three plants; Dioscorea membranacea Pierre ex Prain & Burkill, Dioscorea birmanica Prain & Burkill (Dioscoreaceae) and Siphonodon celastrineus Griff. (Celastraceae), exhibited high cytotoxic activity showing a certain degree of selectivity against the different cell types.

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In vitro cytotoxic activity of Thai medicinal plants used traditionally to treat cancer

Abstract

Keywords

ASJC Scopus subject areas

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