Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

M. Riepe; A. Ludolph; M. Seelig; P. S. Spencer; A. C. Ludolph

doi:10.1097/00001756-199410270-00035

Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

M. Riepe, A. Ludolph, M. Seelig, P. S. Spencer, A. C. Ludolph

Neurology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

Original language	English (US)
Pages (from-to)	2130-2132
Number of pages	3
Journal	NeuroReport
Volume	5
Issue number	16
DOIs	https://doi.org/10.1097/00001756-199410270-00035
State	Published - Oct 1994

Keywords

3-NPA
ATP
Excitoxicity
Glutamate
Glutamate antagonist
Neurodegeneration
SDH

ASJC Scopus subject areas

Neuroscience(all)

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10.1097/00001756-199410270-00035

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title = "Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection",

abstract = "Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.",

keywords = "3-NPA, ATP, Excitoxicity, Glutamate, Glutamate antagonist, Neurodegeneration, SDH",

author = "M. Riepe and A. Ludolph and M. Seelig and Spencer, {P. S.} and Ludolph, {A. C.}",

year = "1994",

month = oct,

doi = "10.1097/00001756-199410270-00035",

language = "English (US)",

volume = "5",

pages = "2130--2132",

journal = "NeuroReport",

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T1 - Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

AU - Riepe, M.

AU - Ludolph, A.

AU - Seelig, M.

AU - Spencer, P. S.

AU - Ludolph, A. C.

PY - 1994/10

Y1 - 1994/10

N2 - Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

AB - Succinic dehydrogenase in mouse cortical explant cultures was inhibited by 3-nitropropionic acid (3-NPA). ATP concentrations declined upon application of 3-NPA. At 4 h, ATP levels of cultures treated with 3-NPA alone were no different from those in cultures treated additionally with MK-801 (20 /xM), 6-cyano-7-nitroquinoxa- line-2,3-dione (CNQX; 10 /uM) or a combination thereof. However, MK-801 and MK-801 plus CNQX mitigated morphological lesions caused by 3-NPA. CNQX alone did not influence the extent of morphological damage. In conclusion, MK-801, at concentrations which were neuro- protective against 3-NPA lesions in cortical explant cultures, did not modify 3-NPA dependent decreases in cellular ATP levels. These data indicate that the neuropro- tective effects of glutamate receptor antagonists in this model are probably receptor mediated and do not involve effects on cellular metabolism.

KW - 3-NPA

KW - ATP

KW - Excitoxicity

KW - Glutamate

KW - Glutamate antagonist

KW - Neurodegeneration

KW - SDH

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AN - SCOPUS:0028151690

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JO - NeuroReport

JF - NeuroReport

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ER -

Increase of ATP levels by glutamate antagonists is unrelated to neuroprotection

Abstract

Keywords

ASJC Scopus subject areas

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