Inhibition of axonal growth by brefeldin A in hippocampal neurons in culture

Mark Jareb, Gary Banker

Research output: Contribution to journalArticlepeer-review

99 Scopus citations


The outgrowth of neuronal processes involves a great increase in the surface area of the cell. The supply of membrane material necessarily must be coordinated with the demands for neurite growth. The selective growth of only one or two neurites at any given time during the development of polarity raises the possibility that the production of materials by the soma is limiting for growth (Dotti and Banker, 1987; Dotti et al., 1988; Goslin and Banker, 1990). To examine the role of the availability of membrane components during the development of polarity and axonal elongation, we treated neurons with brefeldin A, an antibiotic that disrupts the trafficking of vesicles from the Golgi complex to the plasma membrane. Treatment with brefeldin A (1 μg/ml) inhibited axonal growth within 0.5 hr; in unpolarized cells it prevented the formation of an axon. These results indicate that the availability of membrane components of Golgi-derived vesicles is required for axonal growth and hence the development of polarity. Inhibitors of protein and RNA synthesis also blocked axonal growth and the development of polarity, but over a much slower time course. This suggests that the full complement of proteins and mRNAs required for the initial development of polarity is present for several hours before polarity is actually established.

Original languageEnglish (US)
Pages (from-to)8955-8963
Number of pages9
JournalJournal of Neuroscience
Issue number23
StatePublished - 1997
Externally publishedYes


  • Axonal transport
  • Brefeldin A
  • Golgi complex
  • Hippocampal neurons
  • Neurite outgrowth
  • Polarity

ASJC Scopus subject areas

  • General Neuroscience


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